The present work aimed to apply protoplast fusion
of four Trichoderma spp.
Keywords: Somatic hybridization; Embryogenesis; Callus culture; Protoplast fusion
; Liquid medium
Genome shuffling is a useful method for rapid breeding of improved microorganisms and involves the generation of mutant strains that have an improved phenotype, followed by multiple rounds of protoplast fusion
to allow recombination between genomes [7,8].
The use of UV radiation and of protoplast fusion
have resulted in mutant Trichoderma strains, with higher enzyme levels production than parental strains, including chitinases, glucanases and proteases.
have used EMS mutagenesis and interspecific protoplast fusion
to isolate improved probiotic yeasts.
giganteus) by protoplast fusion
using polyethylene glycol (PEG).
studies using polyethylene glycol (PEG) is one of the possible alternatives as it is a powerful tool for genetic improvement of this medicinal plant.
Somatic hybrids obtained by protoplast fusion
between Solanum tuberosum L.
Techniques like protoplast fusion
and genetic modification go a step further than embryo rescue tissue culture.
Sreenath and his team hope that, once they have it, they will be able to use protoplast fusion
to induce the gene fragments of fuse with a more commercially viable cultivar and create the first caffeine-free Arabica or Robusta.