protoplast fusion


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protoplast fusion

the joining together of two or more PROTOPLASTS. Often the presence of polyethylene glycol or electric fields will enhance the frequency of fusion. In the fusion unit the DNA from the component cells may undergo RECOMBINATION. This method is useful in GENETIC ENGINEERING. It may allow the exchange of DNA between unrelated ORGANISMS if they are capable of undergoing protoplast fusion.
References in periodicals archive ?
Tenders are invited for Electroporator for protoplast fusion
The present work aimed to apply protoplast fusion of four Trichoderma spp.
Keywords: Somatic hybridization; Embryogenesis; Callus culture; Protoplast fusion; Liquid medium
Genome shuffling is a useful method for rapid breeding of improved microorganisms and involves the generation of mutant strains that have an improved phenotype, followed by multiple rounds of protoplast fusion to allow recombination between genomes [7,8].
have used EMS mutagenesis and interspecific protoplast fusion to isolate improved probiotic yeasts.
Somatic hybridization by protoplast fusion is a promising technique for breeding ornamental species and requires reliable in vitro protocols.
giganteus) by protoplast fusion using polyethylene glycol (PEG).
Protoplast fusion studies using polyethylene glycol (PEG) is one of the possible alternatives as it is a powerful tool for genetic improvement of this medicinal plant.
Techniques like protoplast fusion and genetic modification go a step further than embryo rescue tissue culture.
Sreenath and his team hope that, once they have it, they will be able to use protoplast fusion to induce the gene fragments of fuse with a more commercially viable cultivar and create the first caffeine-free Arabica or Robusta.