As the CPB duration increased a progressive impairment in platelet function 25 occurred , excessive bleeding after long CPB intervals may be related, in part, to a thrombinmediated consumptive state (systemic thrombin generation has been shown to occur in stepwise, time dependent fashion), since fibrin monomer, thrombin/antithrombin complexes, and prothrombin fragment 1.2
levels increase progressively with time on CPB when ACT-based anticoagulation protocols are used.
measured plasma concentrations of fibrinogen, D-dimer, thrombin-antithrombin III complexes, and prothrombin fragment 1.2
and evaluated the disseminated intravascular coagulation score.
The work-up should also include a prothrombin time/partial thromboplastin time and lupus anticoagulant profile in all of its manifestations; a measurement of protein C, S, and antithrombin III levels; a check for activated protein C resistance; as well as use of Markers of Coagulation and Hemostasis Activation (MOCHA) or your laboratory's equivalent in looking for prothrombin fragment 1.2
, thrombin-antithrombin, antithrombin, fibrin monomers, and D-dimers, Dr.
(7) studied an enzyme-linked immunosorbent assay with use of monoclonal capture antibody for prothrombin fragment 1.2
and used a polyclonal peroxidase labeled detector antibody.
At this stage, prothrombin fragment 1.2
([F.sub.1.2]) is released.