When 0.45% (w/v) proteose
peptone was present, 88 cells showed heavy uptake, 10 medium, and two light.
Soybean meal, Yeast extract, Proteose
peptone, Urea, Potassium nitrate, Ammonium sulfate were evaluated for xylanase production using Aspergillus fumigatus SKF-4.
Name Origin/molecular mass Amino acid sequence E-5-K Residues 1-5 of [alpha]- H,N-EQLTK-COOH lactalbumin/689 g/mol L-16-Y Residues 164-179 of EEN-LKKISQRYQKFALPQY- [alpha]-casein/2011.4 Da COOH N-23-T or Residues 113-135 of [H.sub.2]N- Lactophoricin-I bovine component-3 of NTVKETIKYLKSLFSHAFEWKT (LPcin-I) proteose
peptone -COOH (PP3)/2683 Da Name Total net Total No.
Axenic and Monoxenic cultivation in proteose
peptone yeast extract glucose (PYG) medium
Samples from both sites contained strains capable of growth on crude oil as a sole source of carbon (unconditional strains) along with strains that could degrade oil if proteose
peptone was available in the growth medium (conditional strains).
Tetrahymena thermophila strain B7 was grown axenically in PY medium: 0.75% proteose
peptone (Difco), 0.75% yeast extract (Difco), 1.5% glucose, 1 mM [MgSO.sub.4], 50 [mu]M [CaCl.sub.2], and 100 [mu]M ferric citrate.
The composition of media was: [(N[H.sub.4]).sub.2]S[O.sub.4]-1.4 gm, K[H.sub.2]P[O.sub.4]-2.0 gm, Ca[Cl.sub.2]-0.3 gm, [Urea.sup.*#]-0.3 gm, MgS[O.sub.4].7[H.sub.2]O*-0.3 gm, FeS[O.sub.4].7[H.sub.2]O*-5.0 mg, MnS[O.sub.4].7[H.sub.2]O*-1.6 mg, ZnS[O.sub.4].7[H.sub.2]O*-1.4 mg, CO[Cl.sub.2]-2.0 mg, Proteose
peptone-1.0 gm, Tween 80-1.0 ml, Cellulose-10.0 gm, Distilled water-1000 ml, pH-5.0[+ or -]0.2.
An aliquot of the water sample (100 [micro]L) was plated on Difco Reasoner's 2A agar (0.5 g/L proteose
; 0.5 g/L casamino acids; 0.5 g/L yeast extract; 0.5 g/L dextrose; 0.5 g/L soluble starch; 0.3 g/L dipotassium phosphate; 0.05 g/L magnesium sulfate; 0.3 g/L sodium pyruvate) and incubated at 37[degrees] C under aerobic growth condition for 3 days.
The medium for probiotics from anaerobic bacteria was 10 g proteose
LB [2% (w/v) proteose
peptone and 0.1% (w/v) Bacto yeast extract], wheat and rice grain medium (one boiled rice and wheat grain in 10 ml of distilled water) and Bold-basal salt medium [NaNO3 (0.25 g l-1), CaCl2.H2O (0.025 g l-1), MgSO4.7H2O (0.075 g l-1), K2HPO4 (0.075 g l-1), KH2PO4 (0.175 g l-1), NaCl (0.025 g l-1), EDTA (0.05 g l-1), KOH (0.031 g l-1), FeSO4.7H2O (0.04 g l-1), H2SO4 (0.001 M), H3BO3 (0.01142 g l-1), ZnSO4.7H2O (0.00881 g l-1), MnCl2.4H2O (0.00144 g l-1), MoO3 (0.00071 g l-1), CuSO4.5H2O (0.00157 g l-1) and Co(NO3) 2.6H2O (0.00049 g l-1)], diluted 1:1000 with distilled water, with 5-7 wheat grains (Shakoori et al., 2004).
Peptone yeast-extract glucose (PYG): 100 g proteose
peptone (Difco, Sparks, MD), 10 g yeast-extract (Merck, Darmstadt, Germany), 4.9 g MgS[O.sub.4].7[H.sub.2]O, 5 g sodium citrate.2[H.sub.2]O, 0.1 g Fe[[(N[H.sub.4]).sub.2] [[(S[O.sub.4]).sub.2].6[H.sub.2]O, 1.7 g K[H.sub.2]P[O.sub.4], 1.97 g [Na.sub.2] HP[O.sub.4].7[H.sub.2]0, 45 g glucose, and 0.295 g Ca[Cl.sub.2] in 5 L of distilled water.