proteolytic enzyme

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any protein that acts as a catalyst, increasing the rate at which a chemical reaction occurs. The human body probably contains about 10,000 different enzymes. At body temperature, very few biochemical reactions proceed at a significant rate without the presence of an enzyme. Like all catalysts, an enzyme does not control the direction of the reaction; it increases the rates of the forward and reverse reactions proportionally.

Enzymes work by binding molecules so that they are held in a particular geometric configuration that allows the reaction to occur. Enzymes are very specific; few molecules closely fit the binding site. Each enzyme catalyzes a specific type of chemical reaction between a few closely related compounds, which are called substrates of the enzyme.

Enzymes are given names ending in -ase. In older names, the suffix is added to the name of the substrate, as in amylase, an enzyme that breaks down the polysaccharide amylose. In newer names, the suffix is added to the type of reaction, as in lactate dehydrogenase, an enzyme that converts lactate to pyruvate by transferring a hydrogen atom to nicotinamide-adenine dinucleotide (NAD).
Regulation of Enzymes. The reaction rate of an enzyme-catalyzed reaction varies with the pH, temperature, and substrate concentration. Under physiologic conditions the rates of many reactions are controlled by substrate concentrations. Certain key reactions are controlled by one of three different mechanisms.

In allosteric regulation, the enzyme can bind molecules, which are referred to as effectors, at a site other than the active site, which is referred to as an allosteric site. In many biochemical pathways the enzyme that catalyzes the first reaction in the pathway is inhibited by the final product of the last reaction, so that when sufficient product is present the whole pathway is shut down. This is an example of negative feedback.

Many enzymes are regulated by phosphorylation. A phosphate group is attached to the enzyme by another enzyme, called a protein kinase. When the enzyme is phosphorylated it changes its shape and thus its activity. Phosphorylation activates some enzymes and inactivates others; by this means one protein kinase can control several enzymes.

All enzymes are controlled by their rate of synthesis. Like all proteins, enzymes are synthesized by ribosomes, which translate the genetic information coded in the deoxyribonucleic acid (DNA) of the chromosomes into the specific amino acid sequence of the enzyme. The expression of many genes is controlled by the processes of genetic regulation. Thus, although each cell contains the information to make all of the body's enzymes, it actually makes only those appropriate for its specific type of cell. The synthesis of some enzymes can be induced or repressed by the action of specific hormones, substrates, or products so that the enzyme is produced only when metabolic conditions require its presence.
Inborn Errors of Metabolism. Hundreds of genetic diseases that result from deficiency of a single enzyme are now known. Many of these diseases fall into two large classes. The aminoacidopathies result from deficiency of an enzyme in the major pathway for the metabolism of a specific amino acid. The amino acid accumulates in the blood, and it or its metabolites are excreted in the urine. The lysosomal storage diseases result from deficiency of a lysosomal enzyme and the accumulation of the substance degraded by that enzyme in lysosomes of cells throughout the body. The stored material is usually a complex substance, such as glycogen, a sphingolipid, or a mucopolysaccharide.ƒ

An example of an aminoacidopathy is phenylketonuria (PKU), which results from a deficiency of the enzyme phenylalanine hydroxylase, which converts the amino acid phenylalanine to tyrosine. Phenylalanine accumulates in the blood and phenylpyruvic acid is excreted in the urine. The phenylalaninemia eventually results in mental retardation due to defective formulation of myelin. However, PKU can be detected at birth by a screening test for phenylalanine in the blood, and clinical symptoms can be avoided by strict adherence to a low-phenylalanine diet.

An example of a lysosomal storage disease is tay-sachs disease, which results from a deficiency of the enzyme hexosaminidase A. The stored substance is a sphingolipid, GM2-ganglioside, which accumulates in nerve tissue, causing blindness and mental deterioration. No cure is possible, but antenatal diagnosis can be made by determining hexosaminidase A activity in fetal fibroblasts from an amniotic fluid specimen drawn by amniocentesis. It is also possible to identify carriers (heterozygotes) who are at risk for having children with the disease.
Enzyme Assays. Several enzymes are important in clinical pathology. Enzymes characteristic of a tissue are released into the blood when the tissue is damaged; hence assays of serum enzyme levels can aid in the diagnosis or monitoring of specific diseases. Lipase and amylase levels are useful in pancreatic diseases; alkaline phosphatase (ALP), lactate dehydrogenase (LD), aspartate transaminase (AST or GOT), and alanine transaminase (ALT or GPT) in liver diseases; and LD and creatine kinase (CK) in myocardial infarction. ALP is also released in bone diseases. Many enzymes have different forms (isoenzymes) in different organs. The isoenzymes can be separated by electrophoresis in order to determine the origin of the enzyme. Isoenzymes of LD, CK, and ALP have the most clinical utility.
See accompanying table.
activating enzyme one that activates a given amino acid by attaching it to the corresponding transfer ribonucleic acid.
brancher enzyme (branching enzyme) α-glucan-branching glycosyltransferase: an enzyme involved in conversion of amylose to amylopectin; deficiency of this enzyme causes amylopectinosis.
constitutive enzyme one produced by a microorganism regardless of the presence or absence of the specific substrate acted upon.
debrancher enzyme (debranching enzyme) dextrin-1,6-glucosidase: an enzyme that acts on glucose residues of the glycogen molecule and is important in glycogenolysis; deficiency of this enzyme causes Forbes' disease.
induced enzyme (inducible enzyme) one whose production requires or is stimulated by a specific small molecule, the inducer, which is the substrate of the enzyme or a compound structurally related to it.
proteolytic enzyme one that catalyzes the hydrolysis of proteins and various split products of proteins, the final product being small peptides and amino acids.
repressible enzyme one whose rate of production is decreased as the concentration of certain metabolites is increased.
respiratory e's enzymes of the mitochondria, e.g., cytochrome oxidase, which serve as catalysts for cellular oxidations.
Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved.

proteolytic enzyme

An enzyme that catalyzes the conversion of proteins into peptides.
See also: enzyme
Medical Dictionary, © 2009 Farlex and Partners

proteolytic enzyme

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
Proteolytic enzymes, like papain found in papaya seeds, are some of the most powerful natural substances for breaking down undigested protein in your digestive tract.
Bioactive peptides from caseins released by cold active proteolytic enzymes from Arsukibacterium ikkense.
There was a significant increase in the body weight of the animals when the proteolytic enzyme was added to whey protein, compared to the animals which were fed on whey protein alone.
However, in order to obtain only the embryonic egg measurements, the proteolytic enzymes and protein content of nurse eggs were subsequently discounted, using the following approach.
One ideal solution to this issue would be the use proteolytic enzymes capable to degrade the heat unstable proteins [42].
Table 4: Selection of the second proteolytic enzyme to enhance the efficiency of enzymatic hydrolysis of FoodPro-treated silk fibroin.
Orthopaedics: In a clinical trial, patients with long bone fractures were treated with oral bromelain as a proteolytic enzyme combination containing 90 mg bromelain per tablet after surgery.
Due to proteolytic enzymes, these chemically crosslinked gelatin sponges liquefy in vivo within a week or less and are completely absorbed in four-to-six weeks [38].
It was shown that the presence of carbohydrates (over 1%) in the growth medium of the microorganisms increased (from 2 to 15 times) the proteolytic enzyme secretion, whereas their lack (less than 0.1%) in some cases it suppressed [25].
For example, carnivorous species exhibit a wide range and high activity of proteolytic enzymes to process their high-protein diet, whereas herbivores and omnivores ingest large amounts of carbohydrates (Johnston & Freeman 2005).
Serrapeptase is a proteolytic enzyme originally isolated from the non-pathogenic bacteria Serratia species found in the digestive tract of the Japanese silkworm.
Many of the organisms excrete more than one kind of protease, and the type of proteolytic enzyme formed may depend upon the composition of the medium [16].