protein chip

protein chip

A tool for evaluating very large numbers of proteins (e.g., the entire proteome of an organism) using DNA microarray technology. Uses include the evaluation of interactions between proteins and other molecules; the development of new drugs; and the diagnosis of diseases, such as immunological disorders, in which small concentrations of abnormal proteins or antigens occur in body fluids.

protein chip

an orderly arrangement of proteins, immobilized as spots on a matrix at precise locations, to form an array, analogous to DNA CHIP/MICROARRAY. Protein chips can be fabricated by robotically depositing proteins/antibodies on to the surface of a microscope slide. A labelled (see LABEL protein sample, which may contain many analyte proteins, can be applied to the chip. After incubation sample proteins that bind to their capture proteins on the chip can be detected and identified by locating the position/intensity of the label, for example fluorescence. A protein chip allows thousands of proteins to be studied simultaneously. It can be used, for example, for profiling proteins in cells under different conditions and for studying protein-protein and protein-small molecule interactions (see PROTEOMICS).
References in periodicals archive ?
CEO Krik Angacian first had the idea for a protein chip in college, when he became fed up with the lack of variety there was in protein snacks outside of bars and shakes.
By using protein chip AAH-CYT-G1000 kits, we found that IL-34-stimulated FLS exhibited significantly increased epithelial neutrophil-activating peptide-78 (ENA-78), interleukin-8 (IL-8), growth-related oncogene (GRO), and macrophage chemoattractant protein-1 (MCP-1) compared to the unstimulated control (Figures 5(a) and 5(b)).
We chose the three following protein arrays to compare their performance in assessing the protein levels in kidney tissue: the quantitative protein chip QAH-INF-3 (RayBiotech, Inc., Norcross, GA) and the semiquantitative protein L-series antibody array chips L-507 and L-493 (RayBiotech, Inc., Norcross, GA).
According to the different biological material, the chip can be divided into gene chip, protein chip, cell chip and tissue chip.
It is expected that the laboratory and protein chip segments will experience maximum growth within the microarrays segment, attributable to product advancements and broadening capabilities in drug delivery and diagnostic testing.
In addition to diagnostic kits for detecting Influenza A viruses, its products include the proprietary protein chip system for multi-tumor marker detection (C-12), patented HPV DNA screening product for cervical cancer and screening chips for pulmonary tuberculosis.
The development of protein chip or microarray technology has provided a highly sensitive, high-throughput method for disease diagnosis (8, 9) by facilitating detection--in small amounts of patient samples--of antibodies to antigens from infectious viruses or bacteria (10, 11).
In their efforts to identify suitable biomarkers of the progression from normal cognitive functioning to mild cognitive impairment and AD, researchers have begun to use protein chip arrays whose surfaces have been treated chemically or biochemically so that the arrays can bind only proteins with certain structural or functional characteristics.
Another quantitative MS method is the surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry, which has a quantitative protein chip and it is known as SELDI-TOF-MS-based Protein Chip[R].
Agilent also launched its High-Capacity Enrichment Column Chip for sample loads up to 4 t.II and the Intact Protein Chip. Thermo, through its Fisher acquisition, released the Thermo Scientific Pierce SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) Protein Quantitation Kit, featuring in vivo labeling capabilities.
The immuno sector, dominated by companies such as Bayer Diagnostics [Siemens AG], Abbott Diagnostics and Roche Diagnostics, utilises technologies such as ELISA, Luminex, turbidimetric, immunohistochemistry, point of care, flow cytometry and protein chip technologies.
(Hayward, CA; 206-463-4800) announced that the United States Patent and Trademark Office has issued a broad patent covering a fundamental protein chip technology developed by Peter Wagner, Ph.D., and Steffen Nock, Ph.D., co-founders of Zyomyx, and others, that establishes a broad coverage for creating surfaces ideally suited to the study of protein interactions.