Fungal protease has attracted the attention of experts of environmental biotechnology since fungi can grow on low-cost substrates and secrete huge quantities of the enzyme into the culture medium.
An early stage in the development of industrial fermentation process isolated different organisms for the production of protease in the large-scale (Germano et al., 2003).
Additionally, intestinal digesta contents from the distal end of duodenum to ileo-caecal junction and pancreas samples were collected for protease
89% for males using SBT1; chymotrypsin activity was inhibited by 56% for females and 61% for males when using TPCK; while metal protease
activity was inhibited by 85% for females and 95% for males using EDTA, and 75% for females and 83% for males when using phenanthroline; finally, protease
activity was inhibited in 62% for females and 75% for males using ovalbumin (Fig.
Screening for best protease
producing strain on medium.
Structure of HIV-1 Protease
. Drugs that are designed to attack HIV-1 protease
are among the stellar breakthroughs of modern medicine.
enzymes from microbial sources are mainly categorized into two groups: (i) the pepsin-like enzymes produced by Aspergillus, Penicillium, Rhizopus, and Neurospora and (ii) the rennin-like enzymes produced by Endothia and Mucor spp., such as Mucor miehei, M.
Upon reaching confluence, the medium was changed to growth factor-free medium, and the plates were incubated for an additional 24 h after which the cells were stimulated with Aspergillus protease
for 1 h.
equiseti, and their inhibitory effect on HCV NS3-NS4A protease
using a SensoLyte TM 520 HCV protease
Status, already available in Europe, Canada & South Africa, detects a chronic inflammatory response in the wound, as indicated by elevated human protease
activity (EPA) and was developed to help guide treatment decisions to enable improved outcomes and more cost effective care, added the company.
Doxycycline led to a significant drop in serine protease
activity by week 8, compared with baseline and with the placebo group.
production was assessed at 24-h intervals for incubation times up to 72 h.