At the 5-day time point, fibroblast proliferation, with neoformation of procollagen
and angiogenesis, was observed, but the collagen fibers were not mature, (Fig.
Levels of hyaluronidase (HA) (A), laminin (LN) (B), type III procollagen
(PCIII) (C), and type IV collagen (CIV) (D) are shown.
Histological analysis of whole lungs from these mice revealed that the adoptive transfer of these cells at either day 5 or 14 resulted in a marked reduction in the extent of pulmonary fibrosis compared with untreated bleomycin-challenged lungs at day 21 (Figure 3(c), middle, right, and left panels) and a significant decrease in the expression of both procollagen
3 and fibronectin 1 transcripts in the fibrotic lungs (Figure 3(d)).
The previous study ( in vivo study) conducted by our group demonstrated the isolation of fibroblasts from the uterosacral ligament tissues of POP and non-POP (NPOP) patients by laser capture microdissection. The expression levels of Type I and III procollagen
proteins were significantly lower in the POP group compared with the NPOP group, whereas the opposite was noted for the expression of Mfn2 , and the previous results were published in European Journal of Obstetrics, Gynecology, and Reproductive Biology in 2014.
Propeptide of type 1 procollagen
is predictive of post-treatment bone mass gain in adult celiac disease.
Risteli, "Measurement of aminoterminal propeptide of type I procollagen
(PINP) in serum," Clinical Biochemistry, vol.
(a) BAP, bone alkaline phosphatase; (b) PICP, C-terminal propeptide of type I procollagen
; (c) int-OC, intact osteocalcin; (d) N-mid OC, N-terminal/midregion osteocalcin; (e) OPG, osteoprotegerin; (f) RANKL, receptor activator of nuclear factor kB ligand; (g) CTX, cross-linked C-telopeptide of type I collagen.
An enzyme-linked immunosorbent assay (ELISA) kit for PIP-1 was obtained from Takara (Procollagen
Type I C-Peptide enzyme immunoassay (EIA) Kit; Takara, Shiga, Japan).
Secondary end points were changes in bone turnover markers (BTM) of total procollagen
type 1 aminoterminal propeptide (tP1NP) and the serum [beta]-isomerized C-terminal telopeptide of type 1 collagen ([beta]-CTX) during the same trial period [10,11].
The secretion of reactive oxygen species (ROS), lactate dehydrogenase (LDH), GSH, matrix metalloproteinases (MMPs), procollagen
type I, IL-6 and transforming growth factor-[beta]1 (TGF-[beta]1) were measured by kits.
Polypeptides called procollagen
chains are produced on polyribosomes bound to membranes of RER and translocated into the cisternae and the signal peptide is clipped off.
In addition, researchers also evaluated the expression of procollagen
type I, fibrillin-1, matrix metalloproteinase-1 (MMP-1) and -12, and ultraviolet (UV)-induced DNA damage in artificially UV-irradiated buttock skin before and after treatment.