In the present paper the preprochymosin, prochymosin, and chymosin genes were cloned from RNA which was extracted from the abomasum of a suckling yak using RT-PCR techniques.
In this work, we successfully expressed the yak chymosin, prochymosin, and preprochymosin genes in P pastoris and obtained their active form in the culture supernatants.
Finally, in this study we cloned the preprochymosin, prochymosin, and chymosin gene and expressed the genes in Pichia pastoris.
All heterologous expressed yak preprochymosin, prochymosin, and chymosin exhibit chymosin activity and cause milk-clotting, but the greatest expression in this study was from the heterologous expressed prochymosin gene (14.55 SU/mL).
Synthesis of calf prochymosin (prorennin) in Escherichia coli.
Investigations on the activation of bovine prochymosin. Eur.