An amount of osteoblasts and trabecular length of the primary spongiosa are decreased by 46.1% (p [less than or equal to] 0.05).
Their number is reduced in the primary spongiosa by 20.4% (p [less than or equal to] 0.05).
MH Did Not Change Primary Spongiosa Height of Metaphysis.
Metaphyseal trabecular bone volume was analysed in the secondary spongiosa, which is composed of enlarged mineralized bony trabeculae modelled or remodelled from the primary spongiosa. There were no significant differences in bone volume (BV/TV %) in fetal (Figure 4(a)) or postnatal (Figure 4(c)) offspring of MH compared to control mothers.
However, MH had no effect on tibial growth plate thickness or primary spongiosa heights, two regions that are mainly responsible for bone lengthening and bone mass accumulation before or after birth.
The absence of formation of normal primary spongiosa
in the metaphysis, the presence of a thin seal of bone at the chondro-osseous junction, with abnormal metaphyseal vascular invasion and arrest of endochondral growth, these findings suggest an uncoupling of endochondral and perichondral growth, and thus, dumbbell-shaped morphological structure of the osseous metaphysis seen in these patients.
A region of interest, an area at least 0.5 mm below the growth plate (excluding the primary spongiosa
and trabecular-connected cortical bone), was selected and remained constant for all animals.
The region studied was the secondary spongiosa, excluding the primary spongiosa
0.2 mm distal from the growth plate.