preparative ultracentrifugation


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preparative ultracentrifugation

Ultracentrifugation to purify mixed solutions of proteins. It separates them based on their densities or their sedimentation coeffieients.
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Thus, it was of interest to compare the percentage of total apo A-I in Sm LpA-I particles obtained by our HP-SEC method with the percentage in the d >1.21 kg/L plasma fraction after preparative ultracentrifugation. As can be seen in Fig.
[beta]-VLDL was isolated from one type III patient by preparative ultracentrifugation of serum at a density of 1.006 kg/L at 10[degrees]C and 145 000g/min for 18 h in a Beckman L70 ultracentrifuge using a Beckman 50.4 rotor.
LDL (d, 1.019-1.063 kg/L) isolation was performed by sequential preparative ultracentrifugation in a Beckman Optima TLX ultracentrifuge with a type 100.4 rotor.
For example, great differences were seen when rodent HDL-C concentrations were determined by this assay and by preparative ultracentrifugation (data not shown).
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