preparative ultracentrifugation


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preparative ultracentrifugation

Ultracentrifugation to purify mixed solutions of proteins. It separates them based on their densities or their sedimentation coeffieients.
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The observations reported herein indicate that in Botryllus and other colonial ascidians, only a single preparative ultracentrifugation step through cesium chloride is required for isolation of biologically functional RNA.
For the method comparison we prepared 2-fold dilutions of a standardized preparation of HDL that had been isolated by preparative ultracentrifugation of serum and used those dilutions to measure PON1 activity by both the DU68 and manual plate methods.
For example, great differences were seen when rodent HDL-C concentrations were determined by this assay and by preparative ultracentrifugation (data not shown).
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