Palabras clave: celulosa, predigestion enzimatica, celulasas comerciales, preparados enzimaticos con actividad multiple, extraccion
Reason why in the last few decades, in particular the commercial cellulolytic and the multiple activity preparations have been applied successfully to facilitate and to increase the release of interest compounds or for improving the technological process by means of the enzymatic predigestion of the cell wall of the plant tissues.
Histochemical stains showed negative staining for PAS with diastase predigestion
(dPAS), and in a few epithelial cases, mucicarmine stained focal areas.
Note that in each sample there is either complete loss or marked reduction in 1 of the 2 bands after digestion relative to the predigestion
Samples (50-100 mg) of each plant part were digested by a Kjeldahl procedure using a zirconium copper catalyst (Glowa, 1974) and a salicylic acid predigestion
step to convert [NO.
Immunohistochemical Methodology(*) Antibody Details Pretreatment Dilution ACTH NIH, nACTH-IC-2 Pronase predigestion
1:1200 [Alpha]-Subunit BioGenex, None 1:50 [Alpha]-HCG Bcl-2 Zymed, Bcl-2-100 Microwave antigen 1:50 retrieval [Beta]-FSH Immunotech, Pronase predigestion
After this predigestion
the vessels were sealed and placed in the microwave digester.
Of these antibodies, CD3 and CD23 required predigestion
for 20 minutes with 0.
Segments of nerve and skin were embedded in paraffin, and 4-[micro]m sections were stained with hematoxylin-eosin and periodic acid-Schiff, with and without diastase predigestion.
The inclusions stained strongly with periodic acid-Schiff, even after diastase predigestion.
20] Heat-induced epitope retrieval was carried out following trypsin predigestion
in citric acid monohydrate buffer (10 mmol/L, pH 6.
Staining was unsuccessful in undigested tissue and in slides predigested with trypsin, but was successful with hyaluronidase (25 mg/mL, Sigma) predigestion