An advantage of this technique is that it requires no acid predigestion
of samples and so reduces contamination risk and preparation time.
For these reasons, it is hard to avoid the suspicion that whatever form a military operational net might take, the information it contains is likely to require considerable structure and "predigestion
." Doctrine will also be necessary to impose constraints on who can use the military net, where, when, and for what.
Histochemical stains including periodic acid--Schiff (PAS) with and without diastase predigestion
and mucicarmine, and immunohistochemical studies using antibodies against broad spectrum keratin (1:50; DAKO Corporation, Carpinteria, CA, USA), epithelial membrane antigen (EMA, 1:80; DAKO MS, Glostrup, Denmark), and carcinoembrionic antigen (CEA, 1:50; Novocastra Laboratories, Newcastle upon Tyne, UK) were also performed.
Furthermore, unlike any other family of crustaceans, the remipedes perform an external predigestion
of their victims, injecting them with enzymes through their nail-like modified maxillules.
The public cannot appeal directly to the commissioners, but must instead submit comments to their staff for predigestion
before the information is passed up the ladder.
While present at low levels in normal dairy products, lactotripeptides are inactive within the original milk protein and must be released through enzymatic predigestion
to become effective.
Note that in each sample there is either complete loss or marked reduction in 1 of the 2 bands after digestion relative to the predigestion
Samples (50-100 mg) of each plant part were digested by a Kjeldahl procedure using a zirconium copper catalyst (Glowa, 1974) and a salicylic acid predigestion
step to convert [NO.sub.3] to [NH.sub.4] (Nelson and Sommers, 1973).
After this predigestion
the vessels were sealed and placed in the microwave digester.
Of these antibodies, CD3 and CD23 required predigestion
for 20 minutes with 0.4% pepsin (P-7000, Sigma Chemical Co, St Louis, Mo) in 0.1 mol/L CL buffer solution at pH 2.0 at 40 [degrees] C to 42 [degrees] C.