Clinical examination revealed fever (104 (0)-105 (0)F), increased respiratory rate, dehydration (mean STT<4sec), pale mucous membranes with marked anemia and enlargement of popliteal lymph nodes in six dogs and both popliteal and prescapular lymphnodes in two dogs.
Blood tinged bilateral nasal discharge (08), elevated temperature (05), anorexia (08), pale mucus membrane (06), enlargement of popliteal lymph nodes (06) and both popliteal and prescapular lymph nodes (02), edema of scrotum (04), disseminated intravascular coagulopathy (05) and unilateral epistaxis (01) were clinical manifestations in present study.
An impression was provided from the tissues of feet, tail, ears and any patent lesion,13 popliteal lymph nodes, spleen and liver and stained with Giemsa and studied microscopically for the presence of amastigotes.
Histology: The animals were euthanized and the femoral bones, popliteal lymph nodes, spleen and liver were removed and sections of 5im in thickness were provided and stained with hematoxylin and eosin for histological studies.
Electron microscopy: A small part of the fresh tissue with dimensions of 1mm from the femoral bones, popliteal lymph nodes, spleen and liver of each rodent was provided for ultrastructural study under a transmission electron microscope and screened for the presence of amastigotes.
Physical examination revealed pink mucous membranes, slightly enlarged popliteal lymph nodes
and rectal temperature of 39.
60F), pale mucous membranes, splenomegaly and enlargement of popliteal lymph nodes
The popliteal lymph nodes
were enlarged and palpation of masses on trunk revealed hard masses with smooth edges.