Quantitative RT-PCR showed intensive replication of viral RNA in the popliteal lymph nodes and salivary gland and moderate replication in the brain and spleen.
We isolated infectious viruses using Huh-7 cells and Vero E6 cells from the plasma and popliteal lymph node of cheetah 1 and the spleen, lymph nodes, and brain of cheetah 2 but not from the serum of cheetah 2.
These neurovascular structures presented a descendant trajectory alongside the medial edge of the LGM, surrounded by moderate adipose tissue and the popliteal lymph nodes
. The right inferior limb did not present muscular agenesis neither structural variations.
Clinical examination revealed fever (104 (0)-105 (0)F), increased respiratory rate, dehydration (mean STT<4sec), pale mucous membranes with marked anemia and enlargement of popliteal lymph nodes
in six dogs and both popliteal and prescapular lymphnodes in two dogs.
The samples consisted of biopsy fragments of skin lesions, healthy skin from the scapular region, spleen, liver, and cervical and popliteal lymph nodes
. Infection in these animals was also confirmed by the isolation of L.
An impression was provided from the tissues of feet, tail, ears and any patent lesion,13 popliteal lymph nodes, spleen and liver and stained with Giemsa and studied microscopically for the presence of amastigotes.
Histology: The animals were euthanized and the femoral bones, popliteal lymph nodes, spleen and liver were removed and sections of 5im in thickness were provided and stained with hematoxylin and eosin for histological studies.
Animals were killed with ketamine and xylazine; fragments of regional popliteal lymph nodes
were inoculated in medium culture 199 (Gibco, New York, USA), supplemented with 10% (v/v) fetal bovine serum (Gibco, New York, USA), 1% human urine, 20 mM L-glutamine (Sigma Aldrich Chemic, Steinhein, Germany), penicillin (Sigma, Steinhein, Germany), streptomycin sulphate (Sigma, St Louis, USA) and cultivated at 25 [degrees]C.
Distant nodes include "central" nodes, located in the trunk (eg, mediastinal and para-aortic nodes) and "peripheral" nodes at other distant sites not draining the ocular adnexa (eg, popliteal lymph nodes
Physical examination revealed pink mucous membranes, slightly enlarged popliteal lymph nodes
and rectal temperature of 39.7[degrees]C.
Clinical examination revealed pyrexia (103.60F), pale mucous membranes, splenomegaly and enlargement of popliteal lymph nodes
. The animal was dull and depressed.
The popliteal lymph nodes
were enlarged and palpation of masses on trunk revealed hard masses with smooth edges.