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Polysome analysis was performed as described previously  with slight modifications.
This procedure estimates the average transit time of a ribosome on an mRNA transcript by following the incorporation of radiolabeled amino acids from nascent peptides in the polysome pool to completed peptides in the soluble protein pool (details in Brandis and Raff, 1978, 1979; Hille and Albers, 1979).
polysome: Group of ribosomes functionally related and held together by a strand, presumable mRNA.
Relationship to other species: It is the [Fe.sup.2+]-dominant, -2N2S polysome of the hogbomite group.
Polysome profiling results revealed an increase of association of HO-2 transcript with polysomes during hypoxia.
At the beginning of water absorption, when viable seeds reach 20 to 40% moisture, many physiological reactions are activated, such as respiration, yield of ATP, mRNA synthesis and DNA repair, as well as synthesis of polysomes and proteins (Bewley & Black, 1994).
13 Niksic M, Slight J, Sanford JR, Caceres JF, Hastie ND: The Wilms' tumour protein (WT1) shuttles between nucleus and cytoplasm and is present in functional polysomes. Human molecular genetics 2003; 13(4):463-71.
Given that FUS associates with RNP granules and polysomes in the mouse brain , it is likely that FUS could regulate dendritic and axonal translation.
Cofractionation of PIWI proteins and piRNAs with polysomes has hinted at the possibility of a potential role for PIWI proteins and piRNAs in translational control [110, 145].
Later isolation of mRNAs comigrating with polysomes obtained from the brown fat of mice with IGF2BP2 deletion identified 13 transcripts encoding mitochondrial components .
(2006) found cells with big vacuoles, polysomes, and several organelles well developed and distributed along the cytoplasm.
In vitro incoration of selenomethionine into protein by Vigna radiate polysomes. Plant Physiol., 66: 182-186
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