Ribosome-inactivating and adenine polynucleotide
glycosylase activities in Mirabilis jalapa L.
These bands are caused by stacking interactions between the bases and the helical suprastructure of the polynucleotide
that provide an asymmetric environment for the bases .
The DNA chromosomes within each molecule are composed of two separate polynucleotide
strands, each arranged with 23 pairs of homologous chromosomes.
Studies on DNA repair enzymes known to be impaired by Cd, such as Ogg1 and polynucleotide
kinase, have reported [IC.sub.50] values ranging from 5 to 100 [micro]M (Bravard et al.
Toxic effects include ion displacement and/or substitution of essential ions from cellular sites and blocking of functional groups of important molecules like enzymes, polynucleotide
and essential nutrient transport systems (Nies, 1999).
The digest was dried, reconstituted in distilled water, and labeled with T4 polynucleotide
kinase and [[gamma]-[sup.32]P]ATP.
XRCC1 has no catalytic activity of its own but it acts both as a scaffold and a modulator of the different activities involved in base excision repair by interacting with and bringing together DNA polymerase [beta], DNA ligase III, poly (ADP-ribose) polymerase in the N-terminal, C-terminal and central regions of XRCC1, respectively and polynucleotide
kinase at the site of DNA damage (4,5).
The apparent paucity of variability has forced leprae epidemiologists to turn to the variability that is inherent in short polynucleotide
repeats (VNTR) as a basis of strain typing.
6,307,039 (Method for Analyzing a Polynucleotide
Containing a Variable Sequence and a Set or Array of Oligonucleotides Thereof), 6,770,751 (Method for Analyzing a Polynucleotide
Containing a Variable Sequence and a Set or Array of Oligonudeotides Thereof) and 7,192,707 (Method for Analyzing Polynucleotide
Targets Using Tethered Oligonucleotide Probes).
Separation of two types of adeno-associated virus particles containing complementary polynucleotide
Compound microsatellite primers (see table 2) were end-labelled with [[[gamma].sup.32]P]-ATP and polynucleotide
kinase (MBI Fermentas) using standard procedures (Sambrook, et al.
Twisting: Twist is the number of helical turns in the DNA, i.e., the complete revolutions that one polynucleotide
strand makes about the duplex axis in the particular conformation under consideration that is the number of bases per turn of the helix.