More than 90% of the mutations have been found in 81 bp (507 to 533 codons) core region of RNA polymerase beta
subunit of rpoB gene6,7.
The Xpert MTB/RIF purifies and concentrates mycobacterium tuberculosis bacilli from sputum samples, isolates genomic material from the captured bacteria by sonication and subsequently amplifies the genomic DNA by PCR.6 The process identifies all the clinically relevant rifampicin resistance inducing mutations in the RNA polymerase beta
(rpoB) gene in the mycobacterium tuberculosis genome in a real time format using fluorescent probes called molecular beacons.
This study was conducted to examine whether the association of a proliferation-inducing ligand (APRIL), spermatogenesis associated 8 (SPATA8), platelet-derived growth factor receptor-alpha (PDGFRA), and DNA polymerase beta (POLB) with SLE can be replicated in a Chinese Han population.
DNA polymerase beta gene mutation in human breast cancer.
Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein.
XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP- ribose) polymerase, and DNA ligase III is a novel molecular nick-sensor' in vitro.
Zhang, "Deregulated expression of DNA polymerase beta is involved in the progression of genomic instability," Environmental and Molecular Mutagenesis, vol.
Gorton et al., "DNA polymerase beta is critical for mouse meiotic synapsis," The EMBO Journal, vol.
Consequently, analysis of internal transcribed spacer 1 (ITS1) region and two important genes named RNA polymerase II largest subunit (RPOIILS) and the mitochondrial DNA polymerase beta
(DPOLB), encoding critical biologic functional proteins in kinetoplastida were performed.
This activity was previously demonstrated in polymerase beta
, which is active in a repair pathway called base excision repair (BER).
While working with Klaus Rajewsky at the University of Cologne in Germany, Gu and his colleagues wanted to know how an enzyme called polymerase beta
affected the function and development of white cells.
Role of DNA polymerase beta
in the excision step of long patch mammalian base excision repair.