polymerase


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Related to polymerase: RNA polymerase, Taq polymerase

polymerase

 [pah-lim´er-ās]
an enzyme that catalyzes polymerization.
polymerase chain reaction a rapid technique for in vitro amplification of specific DNA or RNA sequences, allowing small quantities of short sequences to be analyzed without cloning.

pol·ym·er·ase

(pol-im'ĕr-ās),
General term for any enzyme catalyzing a polymerization, as of nucleotides to polynucleotides, thus belonging to EC class 2, the transferases.

polymerase

(pə-lĭm′ə-rās′, -rāz′, pŏl′ə-mə-)
n.
Any of various enzymes, such as DNA polymerase, RNA polymerase, or reverse transcriptase, that catalyze the formation of polynucleotides of DNA or RNA using an existing strand of DNA or RNA as a template.

POLA1

A gene on chromosome Xp22.1-p21.3 that encodes the catalytic subunit of DNA polymerase that plays a key role in initiating DNA replication.

pol·ym·er·ase

(pŏ-lim'ĕr-ās)
General term for any enzyme catalyzing a polymerization, as of nucleotides to polynucleotides, thus belonging to EC class 2, the transferases.

polymerase

Any enzyme that promotes the linkage of a number of similar or identical chemical subunits into repetitive long-chain molecules (polymers), especially of NUCLEOTIDES to form DNA or RNA. Derivation as in POLYMER with the -ase suffix denoting an enzyme.

polymerase

an enzyme that catalyses the joining of DNA or RNA nucleotides.
References in periodicals archive ?
The structural coordinates for human DNA polymerase [alpha] in ternary complex with RNA-primed DNA template and aphidicolin were obtained from the PDB-ID: 4Q5V with 2.50 A resolutions.
Reactions that reached exhaustion within 30 min were calibrated by normalizing the minimum fluorescent value of each progress-curve to zero, and the maximum fluorescent value to the average number of extendable bases per polymerase. Then, the initial slope of the curve corresponds to the average initial rate in nucleotides per second polymerase (nt/s/poly).
Based on the 2.5 kb gene sequence information of Taq DNA polymerase gene (Di et al., 2012), a pair of primers (Taq1: 5'-GGCCGAATTCATGCTGCCCCTCTTTGAGCCC-3') and Taq2: 5'-GGCCGAATTCTCACTCCTTGGCGGAGAGCC-3') were designed, with EcoRI located at each end of the gene.
Several polymerase chain reaction (PCR)-based techniques have been developed for routine clinical malaria diagnosis, e.g.
This study has confirmed the existence of HBV DNA polymerase resistance mutations for NAs in 24 (8.9%) of 269 treatment-naive patients, composed of five primary drug resistance mutations (rtT184G, rtS202I, rtM204I/V, rtN236T, and rtM250V), one secondary resistance mutation (rtL180M), and one codon mutation that might be baseline polymorphisms without any clinical significance (rtV207I).
As it can be seen from the results of electrophoresis, Taq polymerase is capable to effectively incorporate in the sequence of a strand only modified nucleotide 5-(C8 Alkyne)-2'-Deoxyuridine-5'-Triphosphate.
Previous studies have shown that PB1-F2 of the A/WSN/33 strain (WSN;H1N1) upregulates viral polymerase activity in vitro [11].
Therefore, the methods for rescuing virus in vivo with high efficiency and no need for RNA preparation in vitro are urgently established.T7 RNAP, one of single-subunit RNA polymerases, is widely employed to generate high specific activity labeled RNA probes, RNA for in vitro translation, biologically active mRNA, and preparative quantities of defined length RNA by the run off transcription method (Schenborn and Mierendorf, 1985).
In vivo DNA replication methods for most organisms rely on a class of enzymes known as DNA helicases, which act to locally unwind dsDNA to create small ssDNA regions suitable for polymerase activity.
One could suppose that stabilization of the beta-structured state of PB1 N-terminus would result in inhibition of the interaction of this protein with PA, polymerase complex assembly, and reproduction of viral RNA.
By direct sequencing of clones from two independent PCR experiments with Taq polymerase, we observed 99 unique mutations out of >100 kbp of target DNA sequence.
Polymerase Assay for Incorporation opposite 5' -Neighboring Bases of G or Oz.

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