polyclonal antiserum


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polyclonal antiserum

Immunology A Gemische of antibodies with distinct epitope reactivities, produced in response to a broad antigenic stimulus, which may be harvested from a person exposed to a particular pathogen and administered to another whose response to the pathogen is inadequate. See Immune globulin.
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B) Uninfected A549 cell monolayer stained with anti-HEV-C polyclonal antiserum. C) Infected A549 cell monolayer stained with anti-HEV-C polyclonal antiserum.
The effects of polyclonal antiserum against pig APM protein on growth performances and feed intake are showed in Table 1.
For the primary antibody, the polyclonal antiserum was diluted 1:1000 with 0.5% nonfat milk in T-TBS (10 mM Tris-HCl, 150 mM NaCl, and 0.05% Tween 20).
Previous studies have demonstrated that SARS-CoV N protein can cross-react with polyclonal antiserum induced by group 1 animal CoVs (8).
This latter type of interference reportedly could be avoided by either coating the gold particles with the F[(ab').sub.2] fragments instead of the complete IgG (26) or by use of a polyclonal antiserum against RF (27).
A polyclonal antiserum raised against the tail of cloned myosin I[Beta] also detected, on immunoblots, a 120-kD protein of similar abundance (T.
In parallel, immunohistochemical (IHC) testing was carried out on the cell cultures by using the avidin-biotin complex technique, with a polyclonal antiserum raised in mice against WNV antigens, for which cross-reactivity with Usutu virus had been demonstrated previously (1).
Figure 1 shows a transverse section of anemone tentacle stained with polyclonal antiserum raised to NF 200.
An IgG fraction of a goat polyclonal antiserum (International Enzymes, Inc.) and an affinity-purified IgG (SCI-PAC) against human CRP were used.
Clearly limulin and Limulus CRP are closely related: both proteins bind to PE-affinity resins; both have the same native ([similar to]300 kDa--by size exclusion chromatography on Sephacryl S-300 HR resin) and subunit [[similar to]24-28 kDa--by SDS-polyacrylamide gel electrophoresis (PAGE)] molecular masses; and both have the same amino terminal peptide sequence (LEEGEGITSKV), and a polyclonal antiserum produced against purified Limulus CRP cross-reacts with limulin.
A polyclonal antiserum was prepared as follows: rabbits were immunized by intracutaneous injection of 100 [micro]g of total GST-[alpha] in complete Freund's adjuvant and boosted twice by subcutaneous injection of 50 [micro]g of GST-[alpha] in incomplete Freund's adjuvant.