B) Uninfected A549 cell monolayer stained with anti-HEV-C polyclonal antiserum
. C) Infected A549 cell monolayer stained with anti-HEV-C polyclonal antiserum
The effects of polyclonal antiserum
against pig APM protein on growth performances and feed intake are showed in Table 1.
For the primary antibody, the polyclonal antiserum
was diluted 1:1000 with 0.5% nonfat milk in T-TBS (10 mM Tris-HCl, 150 mM NaCl, and 0.05% Tween 20).
Previous studies have demonstrated that SARS-CoV N protein can cross-react with polyclonal antiserum
induced by group 1 animal CoVs (8).
This latter type of interference reportedly could be avoided by either coating the gold particles with the F[(ab').sub.2] fragments instead of the complete IgG (26) or by use of a polyclonal antiserum
against RF (27).
A polyclonal antiserum
raised against the tail of cloned myosin I[Beta] also detected, on immunoblots, a 120-kD protein of similar abundance (T.
In parallel, immunohistochemical (IHC) testing was carried out on the cell cultures by using the avidin-biotin complex technique, with a polyclonal antiserum
raised in mice against WNV antigens, for which cross-reactivity with Usutu virus had been demonstrated previously (1).
The polyclonal antiserum
bound the maximal amount of TRAP when diluted up to 1:1000 (Fig.
Figure 1 shows a transverse section of anemone tentacle stained with polyclonal antiserum
raised to NF 200.
An IgG fraction of a goat polyclonal antiserum
(International Enzymes, Inc.) and an affinity-purified IgG (SCI-PAC) against human CRP were used.
Clearly limulin and Limulus CRP are closely related: both proteins bind to PE-affinity resins; both have the same native ([similar to]300 kDa--by size exclusion chromatography on Sephacryl S-300 HR resin) and subunit [[similar to]24-28 kDa--by SDS-polyacrylamide gel electrophoresis (PAGE)] molecular masses; and both have the same amino terminal peptide sequence (LEEGEGITSKV), and a polyclonal antiserum
produced against purified Limulus CRP cross-reacts with limulin.
A polyclonal antiserum
was prepared as follows: rabbits were immunized by intracutaneous injection of 100 [micro]g of total GST-[alpha] in complete Freund's adjuvant and boosted twice by subcutaneous injection of 50 [micro]g of GST-[alpha] in incomplete Freund's adjuvant.