We produced polyclonal antibody
against a synthetic peptide derived from the constant part of P110.
“All four of the polyclonal antibody
based ELISA kits offered in our VisuLize[TM] line are developed after extensive research and are FDA certified to provide our clients the level of quality that they expect of us.
Bordetella pertussis strain 18323 (ATCC 9797), a good producing PT and highly pathogenic strain (MAST, Germany), E.coli DH5a as cloning host, BL21 (DE3) (Novagen, USA) as expression host, pET-22b(+), pET-14b (Novagen, USA) and pAED4 as expression vectors, SmartTag DNA polymerase, [T.sub.4] DNA ligase, dNTPs (Cinagen, Iran), Pill DNA polymerase, Ndel and Xhol (Fermentas, Lithounia), monoclonal antibody against pertussis toxin (Abeam, USA), polyclonal antibody
against pertussis toxin (NIBSC, England) and HRP conjugated antibody against mouse IgG (Sigma, USA) were used in this study.
The package insert states that the device uses 1 monoclonal antibody and 1 polyclonal antibody
. It is possible that one lot used a new batch of polyclonal antibody
with a more avid specificity for hCG[beta]cf or that it used polyclonal antibody
at a concentration that yielded a lower binding capacity for hCG.
ATG-Fresenius S is a polyclonal antibody
preparation used for T-lymphocyte suppression to prevent organ graft rejection in transplant recipients.
The process used may include adsorption on calcium phosphate/hydroxyapatite, a hydrophobic matrix, a chelate matrix, a matrix on which heparin or a substance related to heparin, such as heparin sulfate or dextran sulfate, is immobilized, or a matrix that is coated with an immobilized monoclonal or polyclonal antibody
directed against the protein to be isolated, or F(ab) or F(ab).sub.2 fragments of antibodies directed against the protein to be isolated.
Kleer and her colleagues tested a polyclonal antibody
stain for RhoC to determine whether cancers staining positive for the protein showed the highest potential for invasion and metastasis.
Proteins were identified with anti-p-85 (subunit of phosphatidyl inositol 3-kinase) polyclonal antibody
, followed by horseradish peroxidase-conjugated secondary antibody and visualized with ECL chemiluminescence reaction reagent (Amersham, Buckinghamshire, England) and Kodak X-ray film (X-OMAT AR; Sigma).
The study tested 900 breast cancer patients for HER-2/neu gene amplification with FISH, and tested the same patients with nonantigen-retrieval IHC with the polyclonal antibody
In addition to the company's polyclonal antibody
work to create rIVIG therapeutics, GigaGen has an internal monoclonal antibody discovery pipeline against 17 distinct immuno-oncology targets.
(7-9) More recent studies have shown that the rabbit polyclonal antibody
employed in these 2011 studies is not completely PAX-8 specific.
Added to that, the hybridoma methodology takes a great deal longer to produce antibodies as contrasted with polyclonal antibody