Specificity factors in cytoplasmic polyadenylation
. Wiley Interdiscip Rev RNA 2013;4(4):437-461.
Primers used for PCR amplifications are given in Tables 1, 2, and 3 for the peregrine falcon, the snowy owl, and the blue-fronted Amazon parrot, respectively, and include the generic polyA primers used to identify the site of 3-prime polyadenylation
. All primers were purchased from ThermoFisher oligo services (ThermoFisher Scientific, Waltham, MA, USA).
In addition, for the first time, the 3' UTRs of the LYT1 mRNAs were delimitated; interestingly, the existence of two types of transcripts, differing in length by 116 nts and generated by alternative polyadenylation
Indeed, TTSa-RNAs have been described as AGO1/2-associated, miRNA-(22-24 nt) sized sRNAs whose 3' ends aligned with the annotated polyadenylation
sites and mapping on the sense strand .
Histone modification refers to the process of histone methylation, acetylation, phosphorylation, polyadenylation
, ubiquitination, and ADP glycosylation under the action of related enzymes.
Nucleic Acid Extraction, RNA Polyadenylation
, and Reverse Transcription of Poly(A) RNA.
Takeuchi et al., "Bidirectional control of mRNA translation and synaptic plasticity by the cytoplasmic polyadenylation
complex," Molecular Cell, vol.
Mitochondrial mRNA stability and polyadenylation
during anoxia-induced quiescence in the brine shrimp Artemia franciscana.
The cDNA sequence contained a stop codon (TGA) at position 805-807 bp and a polyadenylation
signal (AATAAA) located 12 bp upstream of the poly (A) tail (Fig.
allows differential negative feedback of human miRNA miR-579 on its host gene ZFR.
(APA) has been emerged as an important mechanism for posttranscriptional regulation of genes in eukaryotes .
Manley, "Mechanisms and consequences of alternative polyadenylation
," Molecular Cell, vol.