polyacrylamide gel


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polyacrylamide gel

n.
A hydrated polymer consisting of a long chain of amide groups, used as a medium for substances that undergo gel electrophoresis.
References in periodicals archive ?
Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels. Anal Chem 1996;68:850-8.
Silver and cyanine staining of oligonucleotides in polyacrylamide gel. PloS one 2015; 10(12): e0144422.
Gordon, "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications," Proceedings of the National Acadamy of Sciences of the United States of America, vol.
Caption: Figure 1: Silver-stained 6% polyacrylamide gel showing LAMP products from sensitivity test.
Alternatively, an increased resolution of LPS can be obtained with SDS-PAGE using a Tricine buffer system [53], or by employing polyacrylamide gel concentration gradients within the gel [54,55].
For further analysis by SDS-PAGE, 20 [micro]L of individual or two pooled peak fractions were mixed with 4 x SDS loading buffer and loaded onto a 10% polyacrylamide gel. The gel was then stained with colloidal Coomassie [5].
Dakis and Kouretas (2002), for example, have described some modifications in the DD technique applied to fish samples, which were characterized using a combination of longer arbitrary primers (25 and 26-mer) together with 30-mer anchored primer for PCR reactions, and the use of a nondenaturing polyacrylamide gel to analyze PCR products.
Bedford and Reid (1969) also carried out a rudimentary polyacrylamide gel fractionation of amylase in the style extracts.
A set of 6 microsatellite markers analyzed were: OarAE101 OarVH72 MAF33 MM12 ETH152 and OarFCB48 illustrated in Table 1 selected for this study used as unlabeled markers to perform the fragment length analysis of the PCR products using 12% non denaturing polyacrylamide gel
The variation of zein fraction of seed storage protein in maize genetic resources was assessed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE).