Effect of EGCG on the first polar body
extrusion rate of bovine oocytes Concentration NO.
Until 2010, early embryo biopsy was the most frequent aspect of genetic analysis (1999-2009: 26.284 cycles; 2009-2010: 4.918 cycles) compared to polar body
(1999-2009: 3.750 cycles; 2009-2010: 997 cycles) and blastocyst biopsy which was used the least often (1999-2009: 105 cycles; 2009-2010: 6 cycles) .
Number of Groups oocytes Number of GVBD Number of PB1 Control 292 125 91 Normal serum 277 102 117 FSH 275 103 118 Medicated serum 335 133 168 Number of Number of Groups fertilized cleavage embryos Control 46 22 Normal serum 60 37 FSH 67 35 Medicated serum 111 59 GVBD: germinal vesicle breakdowns; PB1: first polar body
; FSH: follicle-stimulating hormone.
[1.] A variety of techniques may be employed in preimplantation diagnosis, including embryo analysis, trophectoderm biopsy, and polar body
The data on percentage of oocytes with expanded cumulus and first polar body
extrusion (FPBE) are presented in Table 1.
At polar body
formation, two polar bodies are formed simultaneously.
The red ooplasm began streaming toward the vegetal pole soon after release of the 1st polar body
, which is consistent with results of experimental work showing that morphogenetic determinants within equally cleaving gastropod eggs become segregated along the animal-vegetal axis between release of the 1st and 2nd polar bodies or between the 2nd polar body
release and 1st cleavage (4).
Headed by another Russian emigre, Yury Verlinsky, Ph.D., RGI offers preimplantation genetic diagnosis with polar body
sampling or blastomere biopsy, and markets its services to couples concerned about passing inherited genetic disorders to their offspring.
During the specialized cell division that leads to the creation of egg cells, the leftover complement of 23 chromosomes gets packaged into a smaller cell called a polar body
, which remains attached to the egg but eventually wastes away.
In the water temperature experiment, the highest percentage of oocytes without a polar body
(PB) occurred at 19[degrees]C, indicating delayed meiotic development, with a nondevelopment rate of 75.93%, even after 25 min.
For enucleation, only oocytes with an excellent morphology, and that had extruded the first polar body
, were used for SCNT.
"We are delighted to announce the world's first pregnancy using real time polar body
array Comparative Genomic Hybridization (CGH) and the baby is due later in the spring," the Telegraph quoted Dr Simon Fishel, from the Care Fertility Group in Nottingham, as saying.