Successful viral replication and integration into the host is also dependent on the expression of the pol gene
products (Shao et al.
Yang and colleagues designed two guide RNAs that target the pol gene
of the retroviruses for cutting.
This study not only gives insights about mutational prevalence of pol gene
of HCMV in Turkey, it also reveals all the polymorphic regions on pol gene
in order to gain access to the most conserved regions on this specific gene.
HIV pol gene
sequences were available for the 30 index patients, but not for their 95 sex partners and social contacts.
HIV-1 pol gene
variation is sufficient for reconstruction of transmissions in the era of antiretroviral therapy.
Genomic sequence comparison and phylogenetic analysis based on amino acids of the pol gene
(2,562 bp) were constructed with ClustalW version 2.
Moreover, the molecular cloning of POL gene
would provide valuable information for further understanding of the structure-function relationship through its expression and mutagenesis.
RT-PCR of the pol gene
of HIV was performed and then sequenced for both the RT and PR regions of interest with the TruGene HIV-1 genotyping kit (Visible Genetics, Atlanta, Georgia, United States of America) according to the manufacturer's instructions.
Ghent, Belgium) has patented a method for detection and/or genetic analysis of HBV in a biological sample, comprising hybridizing the polynucleic acids of the sample with a combination of at least two nucleotide probes, with said combination hybridizing specifically to a mutant target sequence chosen from the HBV RT pol gene
region and/or to a mutant target sequence chosen from the HBV preCore region and/or to a mutant target sequence chosen from the HBsAg region of HBV and/or to a HBV genotype-specific target sequence, with said target sequences being chosen from FIG.
Previous subtyping, based on phylogenetic analyses of pol gene
sequences, showed that sequences from patients TV721 and TV749 grouped together (100% bootstrap) but only loosely with clade J references (61% bootstrap); sequences from patients TV725 and TV919 grouped outside all major clades (6).
Novartis AG (Basel, Switzerland) has patented BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter operably linked to the DNA segment; and a transgene operably linked to a second promoter; b) a BIV packaging vector construct comprising a BIV DNA sequence fragment comprising at least a gag gene or pol gene
of BIV; a promoter operably linked to the BIV DNA fragment; and a polyadenylation sequence located downstream of the BIV DNA fragment; and c) an expression vector construct comprising a gene encoding a viral surface protein.