is made up of individual components that assemble into a doughnut-shaped superstructure that punches a hole in the human cell wall, causing the cell to disintegrate.
Effects of cigarette smoke condensate on pneumococcal biofilm formation and pneumolysin
polymerase chain reaction for diagnosis of pneumococcal pneumonia and empyema in children.
To create their vaccine, the researchers focused on the serotype 3 gene that codes for pneumolysin
, a toxin produced by all pneumococcal strains.
The research, published in the international peer-reviewed journal PLoS Pathogens, found that pneumolysin
, the toxin in the bacteria, also triggers an immune response.
gene encodes the hemolysin species-specific protein toxin produced intracellularly by all clinically relevant pneumococcal serotypes (21).
The purpose of this manuscript is to review recent developments with respect to the role of pneumolysin
in pneumococcal disease.
On the other hand, potential new antigens for vaccine formulations, such as the surface proteins PspA, PspC, PsaA, pneumolysin
, BVH-3, and BVH-11, will also need the establishment of reference values.
DNA detection of the pneumolysin
(ply) gene by real-time PCR was performed according to a published assay (5).
We previously produced a mutant in a serotype 2 strain ([DELTA]PAC) that lacked pneumolysin
(Ply) and pneumococcal surface proteins A (PspA) and C (PspC) and found that mutant to be avirulent in mice.
Diagnosis of bacteremic pneumococcal pneumonia by amplification of pneumolysin
gene fragment in serum.
Eligible patients were identified through notification by attending physicians or by clinical microbiology laboratories when a sterile site sample was pneumococcal culture or pneumolysin