Multiplex real time polymerase chain reaction was done to detect the genes encoding the pneumococcal pneumolysin
using primers shown in Table 2.
The objective of present study is to gain more knowledge about the function of important domain of the toxin pneumolysin
. This study aims to analyze the structural and functional features of pneumolysin
and to investigate the residues involved in its pathogenicity.The major virulence factor of this bacterium is a protein, pneumolysin
, which is the member of thiol-activated cytolysins.
The impaired immune response to pneumococcal antigen pneumolysin
due to HIV infection facilitates bacterial invasion .
Clitheroe et al., "Sensitivities of human monocytes and epithelial cells to pneumolysin
are different," Infection and Immunity, vol.
It has also been shown that pneumolysin
causes microscopic lesions in the myocardium .
Kim et al., "MKP1 regulates the induction of MCP1 by Streptococcus pneumoniae pneumolysin
in human epithelial cells," Molecules and Cells, vol.
Streptococcus pneumoniae, which secretes pneumolysin
that help them to act on the respiratory system of various age groups particularly of children that may be lethal to cause death .
Primers ply-894 and ply-974 were described by Corless et al., (11) targeting a specific region of the pneumococcus pneumolysin
gene (ply) amplifying a 80 bp amplicon.
The pathogenesis of increased susceptibility to pneumococcal infection following RSV infection in mice-model studies has been attributed to RSV G glycoprotein-binding penicillin-binding protein 1a increasing pneumococcal virulence owing to up-regulation of virulence genes, pneumococcal toxin and pneumolysin
. This could lead to an increase in the inflammatory response and bacterial adherence to human ciliated epithelial cultures.
[3,16] was used to modify the toxin of SP, pneumolysin
Effects of cigarette smoke condensate on pneumococcal biofilm formation and pneumolysin
. Eur Respir J 41(2):392-395 (2013); http://dx.doi.org/10.1183/09031936.00213211.