Multiplex real time polymerase chain reaction was done to detect the genes encoding the pneumococcal
pneumolysin using primers shown in Table 2.
The objective of present study is to gain more knowledge about the function of important domain of the toxin
pneumolysin. This study aims to analyze the structural and functional features of
pneumolysin and to investigate the residues involved in its pathogenicity.The major virulence factor of this bacterium is a protein,
pneumolysin, which is the member of thiol-activated cytolysins.
The impaired immune response to pneumococcal antigen
pneumolysin due to HIV infection facilitates bacterial invasion [70].
Clitheroe et al., "Sensitivities of human monocytes and epithelial cells to
pneumolysin are different," Infection and Immunity, vol.
It has also been shown that
pneumolysin causes microscopic lesions in the myocardium [55].
Kim et al., "MKP1 regulates the induction of MCP1 by Streptococcus pneumoniae
pneumolysin in human epithelial cells," Molecules and Cells, vol.
Streptococcus pneumoniae, which secretes
pneumolysin that help them to act on the respiratory system of various age groups particularly of children that may be lethal to cause death [9].
Primers ply-894 and ply-974 were described by Corless et al., (11) targeting a specific region of the pneumococcus
pneumolysin gene (ply) amplifying a 80 bp amplicon.
The pathogenesis of increased susceptibility to pneumococcal infection following RSV infection in mice-model studies has been attributed to RSV G glycoprotein-binding penicillin-binding protein 1a increasing pneumococcal virulence owing to up-regulation of virulence genes, pneumococcal toxin and
pneumolysin. This could lead to an increase in the inflammatory response and bacterial adherence to human ciliated epithelial cultures.
[3,16] was used to modify the toxin of SP,
pneumolysin (PLY).
Effects of cigarette smoke condensate on pneumococcal biofilm formation and
pneumolysin. Eur Respir J 41(2):392-395 (2013); http://dx.doi.org/10.1183/09031936.00213211.