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In order to establish efficient transfection complexes for miR delivery in freshly isolated [CD105.sup.+] hMSCs, miR/PEI complexes with different N/P ratios (2.5, 10, and 33) and miR amounts (5 and 10 pmol) were tested (Figures 2(c) and 2(d)).
Therefore we chose magnetic complexes consisting of an N/P ratio of 10 combined with 5 pmol miR bound to 1 [micro]g/mL MNPs as they showed good transfection values in both freshly isolated and cultured hMSCs .
When 1 pmol recombinant attractin was placed in the seawater without a stimulus animal, 3 of 10 animals (30%) were attracted to recombinant attractin, two animals (20%) went to the opposite arm, and five animals (50%) did neither (Fig.
When 1 pmol of recombinant attractin was placed in the seawater adjacent to A.
Test samples included ASW with nothing added (negative control), ASW with 1 or 10 pmol native attractin added, and ASW with 1 pmol recombinant attractin added.
When 1 or 10 pmol of native attractin was placed in ASW containing two non-laying specimens of A.
When 1 pmol of recombinant attractin was placed in ASW containing two non-laying specimens of A.
brasiliana, the percentage of animal pairs mating as hermaphrodites was significantly increased for 10 pmol attractin at every time point between 20 and 170 min and for 190, 200, 230, and 250 min ([chi square](1) > 3.84 for each; P < 0.05; n = 10); the same was true for 1 pmol attractin at 230, 240, and 250 min ([chi square](1) > 3.84 for each; P < 0.05; n = 10) (Fig.
In that study the rate of oxygen consumption measured with a polarographic oxygen sensor was 84 pmol oxygen [larva.sup.-1] [h.sup.-1], compared to the present values of 197 to 347 pmol oxygen [larva.sup.-1] [h.sup.-1], measured with coulometric respirometry, and 213 pmol oxygen [larva.sup.-1] [h.sup.-1], measured with Winkler titration.
Based on maximum transport capacities [ILLUSTRATION FOR FIGURE 3 OMITTED], glucose would be transported at a rate of 28 pmol [h.sup.-1] for a larva, decreasing to 14 pmol [h.sup.-1] for a juvenile.
After incubation, 1 pmol of the appropriate DNA substrate was added, and reactions were performed as follows: AP endonuclease, gap-filling, and flap endonuclease activities for 10 min at 37[degrees]C; and nick ligation assays for 30 min at room temperature.
These studies revealed that undamaged DNA alone (i.e., in the absence of the heavy metal lead) had little (at 100 fmol) or a more significant (at 1 pmol) effect on Ape1 endonuclease activity (Figure 4).
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