OCT4 is a crucial and dependent determinant of pluripotency
in ES and EC cells (10).
The researchers looked at the molecular signals that control TET activity to understand more about how the activity of the TET enzymes can be manipulated during cellular programming to achieve pluripotency
They identified two distinct patterns of DNA methylation, indicating different stages of reprogramming, and showed that individual cells with the later-stage (pluripotency
) pattern also expressed late pluripotency
The capacity for self-renewal and the pluripotency
of ESCs is known to be controlled by the transcription factors, Nanog homeobox (NANOG), octamer binding transcription factor-4 (OCT4) and sex determining region-Y box-2 (SOX2), and signaling pathways like leukemia inhibitory factor (LIF)-signal transducer and activator of transcription 3 (STAT3) and bone morphogenic protein (BMP)-Mothers against decapentaplegic homolog (SMAD) 1/4/5/8 (Rodda et al.
iPSCs generated from the mouse cells have been shown to possess basic features of pluripotency
like the ability to express stem cell markers, differentiating into various tissue types .
29 in the journal Nature detailed research showing success with a process called stimulus-triggered acquisition of pluripotency
, or STAP.
Haruko Obokata, the leader of the study, and her all female research team reported they have discovered a new phenomenon, known as stimulus-triggered acquisition of pluripotency
Certain stem cells can be easily grown in the laboratory and can turn into any type of cell in the body, which is called pluripotency
Haruko Obokata, 30, stating that the young women had shown great talent and initiative in discovering the new phenomenon, known as stimulus-triggered acquisition of pluripotency
Even though hESCs have been studied extensively over the last decade due to their potential to differentiate into cell-types of potential clinical applications, little is known about the role that splicing plays in the regulation of pluripotency
in these cells.
We confirmed pluripotency
of ciPSCs using the following techniques: (i) immunostaining and quantitative PCR for the presence of pluripotent and germ layer-specific markers in differentiated ciPSCs; (ii) microarray analysis that demonstrates similar gene expression profiles between ciPSCs and canine embryonic stem cells; (iii) teratoma formation assays; and (iv) karyotyping for genomic stability.
The first volume begins with explanation of advances in the field as well as definitions, criteria and standards for 'stemness,' the molecular circuitry underlying pluripotency
in embryonic stem cells and iPS cells, and mechanisms of stem cell self-renewal.