is one of the most abundant matrix proteins in the normal liver and is synthesized by hepatocytes.
Human plasma fibronectin
(HFN; Nacalai Tesque, Inc., Kyoto, Japan) was dissolved in PBS (pH 7.4) at 500 [mu]g/mL.
Sepsis screen in neonates with evaluation of plasma fibronectin
. Pediatr Infect Dis J.
Simpson, "The biochemical and functional heterogeneity of circulating human plasma fibronectin
," Biochemical and Biophysical Research Communications, vol.
Oscarsson, "Surface-dependent conformations of human plasma fibronectin
adsorbed to silica, mica, and hydrophobic surfaces, studied with use of atomic force microscopy," Journal of Biomedical Materials Research, Part A, vol.
PCL ([M.sub.W]: 80 kD) and human plasma fibronectin
were obtained from Sigma Aldrich (Saint Louis, MO) as well as all reagents used in functionalization.
[20.] Hayashi M, Yamada KM (1983) Domain structure of the carboxyl-terminal half of human plasma fibronectin
. J Biol Chem 258: 3332-3340.
Bovine serum albumin, human plasma fibronectin
, and its proteolytic fragments, including the III1-C polypeptide (anastellin), guinea pig liver tissue transglutaminase (tTG), human fibrinogen, gelatin, pooled normal rabbit serum, were purchased from Sigma.
Homocysteine Binds to Human Plasma Fibronectin
and Inhibits Its Interaction with Fibrin.
Hcy also binds the fibrinbinding domain of plasma fibronectin
in vitro, inhibiting its ability to bind fibrin (24), and binds factor Va in vitro, making it resistant to inactivation by activated protein C (27).
Briefly, 60 [micro]l of rat plasma fibronectin
standard (10-2000 ng/ml) or cell lysates were incubated with an equal volume of rabbit anti-rat fibronectin (R & D systems, Minneapolis, MN) diluted 1:2000 in wash buffer at 4[degrees]C overnight.