pkat


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pkat

Abbreviation for picokatal.

pkat

Abbreviation for picokatal.
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The [K.sub.m] and [V.sub.max] values for lipase at its optimum pH 8.0 and temperature 40[degrees]C from Lineaweaver-Burk plot were found to be 0.136 mM and 71.4 pkat [ml.sup.-1], respectively (Fig.
aeruginosa Purification steps Total Total activity (pkat protein (mg) p-nitrophenol) Crude 114420.0 1028.4 [(N[H.sub.4]).sub.2] S[O.sub.4] saturation 0-30% 4000.0 17.1 30-60% 6426.6 20.6 60-80% 2273.3 10.4 DEAE-Cellulose 535.0 0.32 Column Chromatography Purification steps Specific Purification activity (pkat fold p-nitrophenol [mg.sup.-1] protein) Crude 111.3 1.0 [(N[H.sub.4]).sub.2] S[O.sub.4] saturation 0-30% 233.9 2.1 30-60% 312.0 2.8 60-80% 218.6 2.0 DEAE-Cellulose 1671.9 15.0 Column Chromatography Table 2.
In control shoots, PAL activity remained relatively stable (about 170 pkat [mg.sup.-1] proteins) during the cultivation period of day 21.