Moreover, pinacyanol chloride (2) is larger in size and would yield better information on dye polymer interaction.
Pinacyanol chloride (Pcyn) was obtained from Acros media and used as received.
The effect of alcohols namely, methanol, ethanol, 2-propanol and urea on the spectra of the pure dye was studied by measuring absorbance of the pure dye solution at 492 nm in case of acridine orange and at 600 nm in case of pinacyanol chloride in the presence of alcohols and urea.
The absorbances were measured at 492 nm and 457 nm in case of acridine orange and at 600 nm and 486 nm in case of pinacyanol chloride.
The absorption spectra of acridine orange (1) and pinacyanol chloride (2) at various concentrations are shown in figures 2 and 3, respectively.
While in case of pinacyanol chloride-heparin complex the stoichiometry is 1:2 and the binding is at alternate anionic sites.
The structures of acridine orange and pinacyanol chloride are given at Figure 1.