1 mL of 1% picryl chloride in ethanol onto the shaved skin of their abdomens.
EFFECT OF ASTILBIN AND ITS METABOLITE ON PICRYL CHLORIDE-INDUCED CONTACT HYPERSENSITIVITY IN MICE AND IN VITRO TNF-[alpha] AND IFN-[gamma] EXPRESSION
We injected astilbin and 3'-O-methylastilbin once intraperitoneally at the time of challenge with picryl chloride.
We collected lymph node cells from mice 5 days after the picryl chloride sensitization and cultured them with or without TNBS in the presence of astilbin and its metabolite.
Furthermore, the glucuronide metabolite did not show any inhibition of picryl chloride-induced ear swelling (data not shown).
In the immunoglobulin (Ig)E-mediated allergic mouse model, AGE significantly decreased the antigen-specific ear swelling induced by picryl
chloride ointment to the ear and intravenous administration of antitrinitrophenyl antibody.
When a solution of DPPH is mixed with that of a substance that can donate a hydrogen atom, then this gives rise to the reduced form with the loss of this violet color (although there would be expected to be a residual pale yellow color from the picryl group still present).
The antioxidant activity of the flower of Nymphaea stellata was determined by using a method based on the reduction of methanolic solution of colored-free radical 1, 1 di-phenyl-1-2 picryl hydrazyl (DPPH).
The antioxidant activity of various sub- fractions was assessed by measurement of scavenging ability of free radical 2, 2'- diphenyl-1- picryl