Studied drugs were administered subcutaneously on depilated parts of the skin at a dose of 0.0005 mg, 0.001 mg, and 0.002 mg in 0.1 cm3 of physiological saline
to determine the tuberculin units of sensitins.
At induction, bolus 0.5 mg/kg ketamine was intravenously given in 5 mL physiological saline
Connective tissue and large vessels were dissected out using a sterile scalpel blade and the tissue was washed using a sterile physiological saline
solution with the aid of a disposable sterile syringe.
For the blockade experiment, either CXCL4mab (1mg/kg) or physiological saline
were delivered to recipient rats (BN-to-Lewis) through the tail vein once every week for 2 months beginning on POD 5.
According to previous studies , ALI was induced in rats by intraperitoneal (i.p.) injection of lipopolysaccharide (LPS) (20mg/kg Escherichia coli B55:5, Sigma Aldrich, USA) in physiological saline
. Prior to the experiment, rats were restrained from eating and drinking for 8 h.
The bacterial cells were harvested by centrifugation at 5000g for 10 min at 4[degrees]C, and the pellet was washed twice and resuspended in sterile physiological saline
An FD was placed in a plastic dish and 10 mL of the dissolution medium (physiological saline
preheated to 37[degrees]C) was added.
The ampoule of freeze-dried vaccine which was diluted in 1000 ml physiological saline
solution containing 1 ml antibiotic solution was used in the experiment.
In general, the time spent between ovary collection and oocyte recovery should be within 3-4 hours and a 30-33[degrees]C temperature range of the physiological saline
should be maintained.
This study contained four groups: wild-type mice that received physiological saline
, wild-type mice that received moxifloxacin, HERG knockout mice that received saline, and HERG knockout mice that received moxifloxacin.
All the drugs were dissolved in physiological saline
. Dosages were chosen according to previous experiments (Tahsili-Fahadan et al., 2006).
Intravenous (IV) fluid of 3000 cc/m2 was administered as 1/3 physiological saline
solution along with bicarbonate (HCO3) treatment.