measurement: The absorption spectra of the PE samples obtained from the purification steps were measured by Microplate reader Spectra MR (Dynex technologies) with a 1-cm light path.
This manuscript describes a procedure to establish a linear scale in four fluorescence channels (fluorescein isothiocyanate (FITC), phycoerythrin
(PE), allophycocyanin (APC), and Pacific Blue (PB)) of a multicolor flow cytometer.
alpha] and [beta] subunits of phycoerythrin
have been shown to be
The raft is red because the cyanobacteria contain the red light-harvesting pigment, phycoerythrin
Surprisingly, concentrations of phycoerythrin
cells expressing CD69 after subtraction of background fluorescence (cells incubated with PBS-BSA followed by the fluorescein conjugated goat anti-mouse IgG or fluorescein/biotinylated isotype matched antibodies plus phycoerythrin
6] cells/[micro]l) and 10 [micro]l of IgG1- phycoerythrin
The three major phycobiliproteins commonly found in cyanobacterial phycobilisomes are; Phycocyanin (PC), Allophycocyanin (APC) and Phycoerythrin
[PE], allophycocyanin [APC]) is excited by a suitable light source and transfers its absorbed energy to an acceptor fluorophore, which then emits this energy as fluorescence at the acceptor-dye wavelength.
Although several nuclear genes have been identified and partially sequenced, the only identified gene presumed to code for a plastid component is cpeA, the alpha subunit of phycoerythrin
sup] Briefly, peripheral blood cells were stained simultaneously using an antihuman fluorescein isothiocyanate-conjugated lineage cocktail, phycoerythrin
(PE)-conjugated CRTH2, and PE-CY7-conjugated CD127, or appropriate isotype controls (all from BD Pharmingen, San Diego, CA, USA).
As part of this study, we compared antibodies conjugated with FITC or phycoerythrin