This manuscript describes a procedure to establish a linear scale in four fluorescence channels (fluorescein isothiocyanate (FITC), phycoerythrin
(PE), allophycocyanin (APC), and Pacific Blue (PB)) of a multicolor flow cytometer.
2006, "Culture media optimization for growth and phycoerythrin
production from Porphyridium purpureum," Biotechnol.
first to be cultured axenically, have no phycoerythrin
(van der Staay et
The raft is red because the cyanobacteria contain the red light-harvesting pigment, phycoerythrin
Two hours after the challenge, 60 [micro]L diluted suspension of phycoerythrin
labeled (red) beads (2 [micro]m in diameter; Polysciences Inc.
[PE], allophycocyanin [APC]) is excited by a suitable light source and transfers its absorbed energy to an acceptor fluorophore, which then emits this energy as fluorescence at the acceptor-dye wavelength.
Unlike wild type cells, which produce the red pigment phycoerythrin
when exposed to green light, these mutants are turquoise in color, revealing a change in the production of phycoerythrin
Fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD4 and CD14, phycoerythrin
(PE)-conjugated CDS and mouse IgG1 isotype were added into the tubes, then the tubes were incubated at 4 [degrees] C for 30 min in the dark.
New applications include Refined Fuels, Phycocyanin, and Phycoerythrin
in addition to previously announced Chlorophyll, CDOM/FDOM, and Crude Oil.
The three major phycobiliproteins commonly found in cyanobacterial phycobilisomes are; Phycocyanin (PC), Allophycocyanin (APC) and Phycoerythrin
6] splenocytes were sequentially incubated in the following combinations of antibodies (Abs): i) fluorescein isothiocyanate (FITC)-conjugated Armenian hamster antimouse CD69 Ab (BD Pharmingen, San Diego, CA, USA), phycoerythrin
(PE)-conjugated rat anti-mouse CD45R Ab and biotinylated monoclonal anti-mouse Thy1.
was chosen as a control, benign alga because it is easily mass-cultured, and it contains both chlorophyll and phycoerythrin
fluorescence useful for microscopic and flow-cytometric analyses.