Nonstandard abbreviations: CN, calcineurin; CNI, CN inhibitor; CsA, cyclosporin A; PBMCs, peripheral blood mononuclear cells; NK cell, natural killer cell; FITC, fluorescein isothiocyanate; PE, phycoerythrin
; APC, allophycocyanin; HBS, Hepes buffered saline.
 Nonstandard abbreviations: HSPC, hematopoietic stem and progenitor cell; MNC, mononuclear cell; rhP/Fc, recombinant human P-selectin/Fc chimera; HBSS, Hanks balanced salt solution; MRE, microrenathane tube; PE, phycoerythrin
; sP, P-selectin; CFC, colony-forming cell; BFU-E, burst-forming unit, erythroid; CFU-GM, colony-forming unit, granulocyte-macrophage.
 Nonstandard abbreviations: EV, extracellular vesicle; AM, acetoxymethyl; CFSE, carboxyfluorescein succinimidyl ester; di-8-ANEPPS, 4-(2-[6-(dioctylamino)-2-naphthalenyl]ethenyl)-1 (3-sulfopropyl)pyridinium; PKH67, Paul Karl Horan 67; EpCAM, epithelial cell adhesion molecule; CD61, integrin, [beta]3; FITC, fluorescein isothiocyanate; APC, allophycocyanin; SEC, sizeexclusion chromatography; mAb, monoclonal antibody; PE, phycoerythrin
; MESF, molecules of equivalent soluble fluorochrome; PPV, positive predictive value.
Cells were then fixed, permeabilized, and incubated with the monoclonal antibody (mAb) phycoerythrin
(PE) conjugated specifically for IFN-[gamma] (phycoerythrin-conjugated IFN-[gamma] mAb-IFN-[gamma]-PE, clone 45.B3), TNF-[alpha] (phycoerythrin-conjugated TNF-[alpha] mAb-TNF-[alpha]-PE, clone MAb11), and IL-10 (phycoerythrin-conjugated IL-10 mAb-IL10-[alpha]-PE, clone JES3-9D7) cytokines (all from Biolegend, USA).
Red algae contain a pigment called phycoerythrin
which reflects red light, making the algae appear red.
cruentum's carotene has Phycoerythrin
. Pigment composition of algae from the genus Nannochloropsis is characterized by chlorophyll a (not chlorophyll b or chlorophyll c), carotene, violaxanthin and vaucheriaxanthin as major pigments.
The separation process was followed by replacing the eluent with a solution of 10% diethyl ether in petroleum ether to produce 0.76 g (8.9%) chlorophyll (green), 0.035 g (0.41%) [beta]-carotene (yellow), 0.03 (0.35%) phycoerythrin
(orange), and residues.
Binding of G[alpha]F-Ig[gamma] BIO was revealed using phycoerythrin
conjugated streptavidin (SAPE) (Biolegend, Cat #405204).
After washing with DPBS, cells were incubated for 1h in DPBS containing PE-conjugated donkey anti-sheep secondary antibody [Donkey F(ab')2 Anti-Sheep IgG H&L (Phycoerythrin
) preadsorbed; Abcam], washed with DPBS, and mounted as described above.
Cells were characterized for expression of surface markers by using fluorescence-labeled mouse monoclonal antibodies binding specifically to human HLA-DR (phycoerythrin
(PE) fluorescent labeled; Becton Dickinson Biosciences, San Jose, CA, USA) and CD45 (PerCP fluorescent labeled; Becton Dickinson Biosciences).
Abbreviations anti-TPO: peroxidase antibodies BAT: Basophil activation test FITC: Fluorescein isothiocyanate PE: Phycoerythrin
Antibodies used in this study were phycoerythrin
(PE) conjugated anti-CD44 and allophycocyanin (APC) conjugated anti-CD45 and anti-CD19 (all from BD Pharmingen).