Fluorescent Microscopy to Observe the
Photobleached Cells
Differential light induction of nitrate reductases in greening and
photobleached soybean seedlings.
When stimulated, the cell could pulse with light a few hundred times before the GFP gave out (technically,
photobleached).
They are known to be
photobleached when exposed to a light and used in photosensitized tumor therapy (Moan, 1986).
On illumination, many porphyrins in simple solution are
photobleached, i.e.
Burns of Procter & Gamble described the design and development of a new series of
photobleaches based on the silicon phthalocyanine (SiPC) ring system.
It possesses a long wavelength absorption that
photobleaches during curing.
The photocyclized products were then
photobleached using a white light lamp.
A small region of interest (ROI) of a probed sample is
photobleached by a brief exposure to an intense focused laser beam.
Additionally, in most photopolymerization systems, the photoinitiator is
photobleached and the light penetrates more deeply into the sample as the photoirradiation continues.
When larvae whose ASO cells had been stained with DASPEI and exposed for 20 min to fluorescent irradiation and thus
photobleached were placed in a seawater solution of acridine orange, the ASO cells uniquely absorbed the dye and emitted a green fluorescence (Fig.
The tissue was then
photobleached by an exposure of approximately 30 min to white light and re-measured.