phosphotungstic acid


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phos·pho·tung·stic ac·id (PTA),

(fos-fō-tŭng'stik as'id),
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.

phos·pho·tung·stic ac·id

(PTA) (fos'fō-tŭng'stik as'id)
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.

phos·pho·tung·stic ac·id

(PTA) (fos'fō-tŭng'stik as'id)
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.

phosphotungstic acid (PTA),

n a mixture of phosphoric and tungstic acids used with hematoxylin for staining muscle tissue and cell nuclei. It is also used as a negative stain of collagen in electron microscopy.
References in periodicals archive ?
Serum TC was done using kit AGAPPE diagnostics, TG using Enzyme calorimetric test and HDL-C using Phosphotungstic Acid method.
Because aqueous uranyl acetate and phosphotungstic acid differ in staining properties, both stains should be applied in parallel in case of unknown samples.
A technique using precipitation of the bands with phosphotungstic acid after agarose gel electrophoresis was reported to allow more reliable quantification of lipoprotein cholesterol but gives discrepant results in samples with atypical lipoproteins (33).
Ten samples of the coronary arteries; 2 of the atria; 8 of the anterior, lateral, and posterior left ventricle; and the anterior and posterior interventricular septum were taken for histologic examination and stained with hematoxylin-eosin and phosphotungstic acid hematoxylin.
Principle: Phosphotungstic acid is reduced by uric acid in presence of sodium carbonate (alkaline medium) to give a blue complex (tungsten blue) which absorbs light at 700 nm.
Bacteria were negatively stained for 2 min with 1% phosphotungstic acid (pH 7.
Serum triglycerides was estimated by glycerol-3-phosphate oxidase method (11), HDL was estimated by phosphotungstic acid precipitation method (12), LDL and VLDL were calculated by Friedewald's formula as follows (13):
Briefly, 50 [micro]L of reagent I (80 g/L phosphotungstic acid and 10 mmol/L EDTA in 0.
Most frequently, HDL-C is measured in the supernatant after precipitation of apolipoprotein (apo) B-containing lipoproteins by dextran sulfate or phosphotungstic acid (PTA)/Mg[Cl.

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