phosphotungstic acid


Also found in: Acronyms, Encyclopedia, Wikipedia.

phos·pho·tung·stic ac·id (PTA),

(fos-fō-tŭng'stik as'id),
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.

phos·pho·tung·stic ac·id

(PTA) (fos'fō-tŭng'stik as'id)
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.

phos·pho·tung·stic ac·id

(PTA) (fos'fō-tŭng'stik as'id)
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.
References in periodicals archive ?
The phosphotungstic acid procedures (Table 4) have been described with and without Mg[Cl.sub.2], adjusted to different pH values, and at several concentrations (42, 53).
The most common negative stains are 1% (60 mM) aqueous uranyl acetate, pH 2-4.5, and 1% (2.5 mM) phosphotungstic acid, pH adjusted to 7.0 with NaOH.
For plasma, 10 [micro]L was placed in a microcentrifuge tube; 500 [micro]L of 42 mmol/L [H.sub.2]S[O.sub.4] was then added and mixed gently, after which 125 [micro]L of 100 g/L phosphotungstic acid was added and vortex-mixed.
HDL-C was isolated and concentrated by precipitating the VLDL and LDL particles from a serum pool with a mixture of 0.44 mmol/L phosphotungstic acid and 20 mmol/L Mg[Cl.sub.2] at a ratio of 1:3.5.
We compared these homogeneous HDL-C assays with our present method (phosphotungstic acid without ion precipitation) by analyzing patient serum samples in parallel and in five separate analyses within 20 days.
Most frequently, HDL-C is measured in the supernatant after precipitation of apolipoprotein (apo) B-containing lipoproteins by dextran sulfate or phosphotungstic acid (PTA)/Mg[Cl.sub.2] [11-14].
Each procedure is representative of one of the three HDL-C assay generations: a phosphotungstic acid (PT)/[Mg.sup.2+] (first-generation) protocol, the modular Spinpro[R] (Sigma Diagnostics, St.
The phosphotungstic acid haematoxylin (PTAH) staining is useful to exhibit numerous small, dark blue to black granule of mitochondria.
(15) Sample was deproteinised by adding phosphotungstic acid and centrifuged at 3000 rpm for 10 minutes.

Full browser ?