Expression of acetyltransferases, nucleotidyl transferases and phosphotransferases
(enzymes related to aminoglycoside-modifying and aminoglycoside resistance), are common too [38-65].
The second most common types of AME were phosphotransferases
, followed by nucleotidyltransferases in which aph(3)-IV and ant(3)-I were detected among 50% (33/66) and 44% (29/66) of isolates, respectively.
Domain search results through SMART program (http://smart.embl-heidelberg.de) confirmed that the horse CK-M domain function is related SMART to enzyme adenosine triphosphate guanido phosphotransferases
. Phylogenetic analysis of the equine CK-M gene with the Neighbor-Joining method showed that the pig and the rodent were, respectively, the closest and the furthest species (Figure 2).
In the final steps, the CDP-choline and the CDP-ethanolamine derivatives are condensed with diacylglycerol, catalysed by multiple DAG-choline and DAG-ethanolamine phosphotransferases
(CPT, EPT, CEPT), to release CDP and to produce the bilayer forming phospholipids PC and PE at the endoplasmic reticulum (ER).
Miziorko, "Identification of active site residues in mevalonate diphosphate decarboxylase: implications for a family of phosphotransferases
," Protein Science, vol.
aminoglycoside acetyltransferases (AACs), aminoglycoside nucleotidyltransferases (ANTs), and aminoglycoside phosphotransferases
(APHs), each of which will chemically convert one or more substituents of the aminoglycosides (Fig.