HygR (hygromycin
phosphotransferase gene), OsSERK- Orysa sativa Somatic Embryogenesis Receptor like Kinase Gene; mGFP5-GusA fusion.
Sanders, "Origin of exopolyphosphatase processivity: fusion of an ASKHA
phosphotransferase and a cyclic nucleotide phosphodiesterase homolog," Structure, vol.
The selection markers were: PGK, mouse phosphoglycerate kinase promoter; NEO, neomycin
phosphotransferase; HPRT, mouse hypoxanthine phosphoribosyltransferase; TK, human herpes simplex virus type 1 thymidine kinase promoter; MT, mouse metallothionein promoter; loxP, a 34 bp recombination site from phage P1.
In the third reaction, CDP-ethanolamine condenses with diacylglycerol to yield PE via the action of integral membrane enzyme 1,2-diacylglycerol ethanolamine
phosphotransferase (EPT).
First, the plasmid pSilent-1 [24] was used as template to amplify the ORF and terminator of hygromycin B
phosphotransferase gene hph (conferring hygromycin B resistance) with primers HygxhoI and Orfhyg.
Inhibition of glucose transport by downregulating Phosphoenolpyruvate
phosphotransferase and [H.sup.+]/ATPase membrane enzymes with decreasing proton motive force is well explained, but still inhibition of enolase enzyme in glucose metabolism by bacteria to form lactic acid is most accepted mechanism of action for fluoride.
It also contains the selectable marker genes hygromycin
phosphotransferase (hyg) and kanamycin (kanr).
They were obtained by using EHA-105 strain of Agrobacterium harboring pBIH binary vector that contained DREB1A controlled by an inducible promoter Lip9 and hygromycin
phosphotransferase gene (hpt) as a plant selectable marker.
Negative controls for these experiments included the use of a non-relevant transcript probe (Neomycin resistance
phosphotransferase gene).
This activation reflected
phosphotransferase activity.
GCV resistance of HCMV is confined to mutations in the viral
phosphotransferase gene (UL97) (7-9) and, less frequently, in the DNA polymerase gene (UL54) (10).
The Cys607-->Tyr change in the UL97
phosphotransferase confers ganciclovir resistance to two human cytomegalovirus strains recovered from two immunocompromised patients.