Kinetics of the bienzyme complex from Chlamydomonas reinhardtii and hysteretic activation of chloroplast oxidized phosphoribulokinase
," European Journal of Biochemistry, vol.
litorea nuclear gene prk, which encodes the Calvin cycle enzyme phosphoribulokinase. Prk was chosen from among the variety of possible genes because several previous studies have found evidence for its horizontal transfer between V.
Molecular characterization of the Calvin cycle enzyme phosphoribulokinase in the stramenopile alga Vaucheria litorea and the plastid hosting mollusc, Elysia chlorotica.
Phosphoribulokinase in two protein forms and an uncharacterized protein GO annotated for proteolysis were both detected in spot 5.
The th-mercaptoethanol extraction method did not appear to break down phosphoribulokinase as severely.
Two genes were selected to provide adequate resolution and taxa coverage: low-copy nuclear gene phosphoribulokinase
(PRK) and chloroplast gene ribulose-bisphosphate carboxylase (rbcL).
Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO) and ribulose 5-phosphate kinase (phosphoribulokinase
) are the marker enzymes of this pathway, and the cycle runs unidi-rectionally to produce 3-phosphoglycerate as a key intermediate.
Two low-copy nuclear DNA regions have been particularly successful: Intron 4 of phosphoribulokinase (PRK), a regulatory enzyme of the Calvin cycle for carbon dioxide assimilation, and intron 23 of RNA polymerase li subunit 2 (RPB2), an enzyme complex responsible for DNA transcription of protein-coding genes in all eukaryotes.
Molecular phylogenetics of tribe Geonomeae (Arecaceae) using nuclear DNA sequences of phosphoribulokinase and RNA polymerase II.
(Akazawa et al., 1978; Kremet et al., 1982;