1995) 'A model for glycolytic oscillations based on skeletal muscle phosphofructokinase
kinetics', Journal of Theoretical Biology, Vol.
2] of modulators of phosphofructokinase
Other glycogen storage diseases, such as deficiencies of phosphofructokinase
and debranching enzyme, would also yield an abnormal Ischemic exercise response.
A partial deficiency of phosphofructokinase
in the red blood cells results in the breakdown of those cells and an increase in blood levels of bilirubin, though the person usually experiences no symptoms.
Assays used to measure ATP-linked fructokinase, UTP-linked fructokinase, glucokinase, sucrose synthase, PPi-linked phosphofructokinase
, ATP-linked phosphofructokinase
, and AGPase were described by Singletary and Below (1990), with the modification of 40 mM UDP for the sucrose synthase reaction mix.
1970), adenylate kinase (TVB), a-glycerophosphate dehydrogenase (TC), glycero-3-phosphate dehydrogenase (TC), glucose phosphate isomerase (CT), homoserine dehydrogenase (TVB), malic enzyme (TC), peptidase (Val-Leu) (RW), phosphofructokinase
(TC), superoxide dismutase (CT), diaphorase (RW), glutamate dehydrogenase (TVB), peptidase (Leu-Gly-Gly) (RW), pyruvate kinase (TVB), and triose phosphate isomerase (TC).
It has also been shown that the AD brain has decreased activity of pyruvate dehydrogenase complex which generates acetyl-CoA, the precursor of acetylcholine from pyruvate [41, 42], decreased activity of phosphofructokinase
, and alpha-ketoglutarate dehydrogenase  but increased cerebral release of ammonia and amino acids [39, 40].
(PFK) activity was determined using an assay kit (Sigma, USA) by a coupled enzyme assay, in which fructose-6-phosphate and ATP were converted to fructose-1,6-diphosphate and ADP by PFK.
A decrease in fructose-2,6-bisphosphate levels inhibits the activity of the rate-limiting enzyme phosphofructokinase
I (PFK1), thereby blocking glycolysis and promoting the shuttling of metabolites to the pentose phosphate pathway (PPP).
The mode of action of meglumine antimoniate has not been determined yet; but the evidence for inhibition of glycolysis in the parasite at the phosphofructokinase
reaction has been found.