Cutting DNA: Endonucleases destroy internal phosphodiester
bonds in the DNA molecule.
9,25) Deoxyribonucleases (DNases) are enzymes that hydrolyse, or break down, the phosphodiester
bonds between the nucleotides in a strand of DNA.
The incorporation of a nucleoside analog into a growing DNA chain aborts DNA synthesis, as phosphodiester
bridges can no longer be built to stabilize the double strand.
Usually the oligonucleotide has N3P5 phosphoramidate or N3P5 thiophiosphoramidate linkages rather than phosphodiester
AO are short (17- to 27-nucleotides long) synthetic DNA molecules with phosphorothioate instead of the naturally occurring phosphodiester
linkages, for in vivo nuclease resistance.
To define the reaction sites of ribozymes, 5' cyanine dye-labeled RNAs were partially hydrolyzed by lead-induced phosphodiester
bond cleavage and fractionated by denaturing PAGE.
The demonstrated safety profile is believed to be related to the high degree of specificity inherent in the structure of LJP 394, which consists of 97% native phosphodiester
dsDNA," he said.
Ribozymes, in particular hammerhead ribozymes, are small catalytic RNAs that specifically cleave phosphodiester
bonds of single-stranded target RNAs.
bond is formed when carbon atoms are linked by a triphosphate group; and by linking nucleotides together through this bond, single-stranded (ss) DNA is formed [ILLUSTRATION FOR FIGURE 1 OMITTED].
increased hydrogen-bonding of the phosphodiester
groups of nucleic acids; 2.
Photo: From an NMR spectrum, Roth's group can measure the levels of these phosphate compounds: phosphocreatine (PCr), adenosine triphosphate ( , , ), inorganic phosphate (Pi), phosphomonoester (PME) and phosphodiester
Exotic DNAs Made of Nonnatural Bases and Natural Phosphodiester