After washing 3 times for 10 min each in PBS, membranes were incubated with rabbit anti-human asynuclein, caspase-3, parkin, phospho-Akt, phospho-GSK3[beta], and phospho-Tau antibodies, respectively, at 1:1000 concentration each overnight at 4[degrees]C.
The levels of [alpha]-synuclein, caspase-3, and phospho-Tau in WT-[alpha]-Syn and A53T-[alpha]-Syn groups were increased significantly, and the levels of parkin, phospho-Akt, and phospho-GSK3(3 in WT-[alpha]-Syn and A53T-[alpha]-Syn groups were decreased significantly, compared with those in control group (p<0.
In the present study, EAS could increase the levels of phospho-Akt and phospho-GSK3[beta] and protect neuronal cell against phospho-Tau activity, which is associated with apoptosis and contribute to the brain damage (Crespo-Biel et al.
They also found that abnormalities associated with ApoE4 and AD, such as the creation of phospho-tau
and amyloid-beta, could be prevented by increasing SirT1, and have identified drug candidates that exert the same effect.
It has been shown in cell cultures and in a number of mice models to reduce and normalize the synthesis of amyloid-[sz] precursor protein (APP) and A[sz], tau and phospho-tau
as well as alpha-synuclein; proteins that are all elevated in the injured brain and that have all been shown to kill nerve cells.
The Buck group also found that the abnormalities associated with ApoE4 and AD, such as the creation of phospho-tau
and amyloid-beta, could be prevented by increasing SirT1.
An abnormal form of the tau protein known as phospho-tau (P-tau) forms into tangles which are the other established lesions in the brain of people with Alzheimer's.
Delphine Boche, PhD, of the University of Southampton's School of Medicine, UK, and colleagues studied the levels of beta amyloid and phospho-tau in six regions of cerebral grey matter that are affected by Alzheimer's pathology in the brains of 10 people with Alzheimer's who were immunized with AN1792 and compared the findings with 28 unimmunized Alzheimer cases.
Biomarkers of neuronal injury, including levels of CSF tau and phospho-tau
These deposits contain the toxic proteins Ab42 and phospho-Tau
Transient increase in total tau but not phospho-tau
in human cerebrospinal fluid after acute stoke.
These new results point to an important connection of NTP to phospho-tau
accumulation in Alzheimer's disease.