Continuously, on MEA vesicle produces simultaneous matula and phialide
(all matula on the surface of vesicle developed in the same time).
Fungal identification was based on macroscopic colony characteristics, and microscopic spore and phialide
characteristics under oil emmersion at 1000x (Bamett and Hunter 2003).
Variation in Metarhizium, a genus of fungal pathogens attacking Orthoptera: Is phialide
morphology a useful criterion.
Classification of the endophytes into taxons was based on phialide
and conidia morphology, and growth in culture.
Variation in Metarhizium a genus of fungal pathogen attacking Orthoptera: is phialide
morphology a useful criterion J.
fujikuroi viz., moniliformis, subglutinans, fujikuroi and intermedia basing on mating population, ascospores, perithecia size, phialide
type and microconidial formations.
Morphological Key Morphological Observation Species Character Colony growth rate (cm) 7-8 cm Colony colour Dark yellowish green Reverse colony colour Colourless Colony edge Smooth Culture smell No characteristic odour Mycelial form Smooth Mycelial colour Cottony white Cultural characteristic Cultural Character Species Character Conidiation Ring like zones Conidiophore branching Branched, regular Phialide
shape Cyllindrical Phialide
size 6.2-10.5 x 3.1-3.9 mm Conidial shape Globose to subglobose Philospore size 2.6-3.0 x 2.0-2.4 mm Conidial wall Smooth Conidial colour Light green Chlamydospore present Spore Germination time 12 -13 Hours
Morphological and cultural characteristics of Trichoderma reesei Morphological characteristic Cultural characteristic Features Species Features Species characters characters Colony growth 5-6cm rate (cm) Colony colour Pale green Conidiophore Highly yellow branching branched Reverse colony Light lemon Phialide
shape Legeniform colour yellow Colony edge Smooth reguler Phialide
size 6.8-9.2x surface 1.9-2.1mm Culture smell No Conidial shape Enlarged in characteristic the middle or smell sub-globose Mycelial form Tufted, Crysty Philospore 2.6-3.0 x size 2.8-3.4mm Mycelial Light yellow Conidial wall Smooth colour Conidial Slight green colour Chlamydospore No Spore 12 Hours Germination time Table 3.
Morphological characterizations including mycelial color, colony texture and shape) and microscopic observations (conidia shape, conidia color, conidiophore--branching, phialide
width and phialide
length were determined according to Sharma and Singh 2014, Singh A, et al 2014.
cylindrical, 8.2-19.2 x 2.1-3.5 (= 11.9+-3.3 x 2.5+-0.4) um.
Metulae covering 1/2 of the whole surface of the vesicle, phialides
4.8 - 17.5 x 2.4 - 6.7 um in size.
B) aspergillus flavus conidiophore bearing vesicle (phialides
arise circumferentially from the globose vesicle) in Lactophenol cotton blue (LPCB).