The single modification was that host tissue homogenates were prepared in ice-cold 0.01 M phosphate buffer containing 400 mM NaCl (pH 8.3) and 0.1 mM phenylmethyl
sulfonyl fluoride (PMSF).
Synthesis and structure-activity relationships for 4-[(phenylmethyl
)amino]- and 4-(phenylamino)quinazolines as potent adenosine 5;-triphosphate binding site inhibitors of the tyrosine kinase domain of the epidermal growth factor receptor," Journal of Medicinal Chemistry, vol.
THE EFFECT OF [GA.sub.4+7] + N-(PHENYLMETHYL
)-AMINOPURINE ON GERMINATION AND EMERGENCE OF Passiflora cincinnata MAST.
A fused-silica capillary column coated with HP-5 cross-linked 5% phenylmethyl
silicone (SE-54, 34 m x 0.2 mm i.d., 0.33 p.m film thickness) was also used.
The clearance of trypsin requires its protease activity, because phenylmethyl
sulfonyl fluoride-inactivated FITC-labeled trypsin was cleared only very slowly if at all ([t.sub.1/2] [is greater than] 180 min).
Frozen rabbit lung tissues were homogenized on ice in CEB buffer (10 mM Tris, pH 7.9, 60 mM potassium chloride, 1 mM EDTA, and 1mM dithiothreitol) containing an antiprotease cocktail (1 mM 4-(2-aminoethyl) benzene sulfonyl fluoride, 0.8 [micro]M aprotinin, 20 [micro]M leupeptin, 50 [micro]M bestatin, 10 [micro]M pepstatin A, 15 [micro]M L-trans-epoxy succinyl-leucyl-amido (4-guanidino) butane, 1 mM phenylmethyl
sulfonyl fluoride, 1 mM sodium fluoride, and 1 mM vanadyl sulfate.
model HP-5MS, coated with 50 mL/L phenylmethyl
siloxane as stationary phase.
20 mM Tris (base pH 7.4), 137 mM NaCl, 10% glycerol, and 1 mM phenylmethyl
We homogenized samples for the determination of enzymatic activities in 5 volumes of 120 mM KCl, 30 mM phosphate buffer (pH 7.2) with added protein inhibitors (1 [micro]g/mL leupeptin, 1 [micro]g/mL aprotinin, 10 [micro]g/mL soybean trypsin inhibitor, 1 [micro]g/mL pepstatin, and 0.5 mM phenylmethyl
sulfonyl fluoride) at 0-4[degrees]C.
Cells were washed twice with ice-cold PBS and lysed in a buffer containing 1% NP-40, 50 mM Tris base, pH 8.0, 50 mM NaCl, 50 [micro]g/ml Leupeptin and 1 mM phenylmethyl
Fish smaller than 90 mg were euthanized in ice-cold MS-222 (0.1 mg/mL) and homogenized in ice-cold phosphate-buffered saline (pH 7.3) supplemented with 20 [micro]M leupeptin, 1 mM phenylmethyl
sulfonyl fluoride (PMSF), 5 mM dithiothreitol, and 5 [micro]g/mL aprotinin.
Chiu PJS, Gerhart C, Brown AD, Baenett A (1984) Effects of gastric antisecretory-cytoprotectant, 2-methyl-8(phenylmethyl
) imidazole (1,2-a) pyridine-3-acetonitrile on cycteamine, reserpine, and stress ulcers in rats.