phenylisothiocyanate


Also found in: Acronyms.

phen·yl·i·so·thi·o·cy·a·nate (PITC, PhNCS),

(fen'il-ī'sō-thī'ō-sī'ă-nāt),
A reagent that condenses with the free N-terminal amino group of a peptide chain to form a phenylthiohydantoin in the Edman method of identifying N-terminal amino acids.
Synonym(s): Edman reagent

Edman,

Pehr, Australian scientist, 1916-1977.
Edman method - a method of identifying N-terminal amino acids.
Edman reagent - used in the Edman method of identifying N-terminal amino acids Synonym(s): phenylisothiocyanate
References in periodicals archive ?
[11.] Cohen SA and DJ Strydom Amino acid analysis utilizing phenylisothiocyanate derivatives.
(4) Nonstandard abbreviations: AIP, acute intermittent porphyria; PBGD, porphobilinogen deaminase; PBG, porphobilinogen; ALA, 5-aminolevulinic acid; MS, mass spectrometry; PITC, phenylisothiocyanate; PTC-PBG, phenylthiocarbamyl porphobilinogen; PTC-ALA, phenylthiocarbamyl 5-aminolevulinic acid; and [M.sub.r], relative molecular mass.
Amino acid analysis by reverse-phase high performance liquid chromatography: Pre-column derivatization with phenylisothiocyanate. Anal.
Cystine was determined as cysteic acid and methinonine as methionine sulfone after preoxidation with performic acid and pre-column derivation using phenylisothiocyanate (L-8800 Hitachi Automatic Amino Acid Analyzer, Tokyo, Japan).
Amino acids in CSF can be determined either by postcolumn derivatization with ninhydrin or o-phthalaldehyde (OPA) by commercially available amino acid analyzers or by precolumn derivatization with different reagents, such as dansyl chloride, phenylisothiocyanate, fluorenylmethyl chloroformate, dabsyl chloride, and OPA, followed by HPLC separation (4-6).
Cystine was determined as cysteic acid and methionine as methionine sulfone after preoxidation with performic acid and pre-column derivation using phenylisothiocyanate (L-8800 Hitachi Automatic Amino Acid Analyzer, Tokyo, Japan).
The more usual methods involve precolumn derivatization with phenylisothiocyanate (PITC) (11,12) or o-phthalaldehyde (OPA) (7,13).
Several methods are suitable for the determination of amino acids (AAs) in biological fluids, including gas chromatography [1,2], reversed-phase chromatography with precolumn derivatization with various reagents such as orthophthalaldehyde (OPA) [3-6], 9-fluorenylmethyl-chloroformate [7], phenylisothiocyanate (PITC) [8, 9], dimethylaminonaphthalenesulfonyl chloride [10], dimethylaminoazobenzenesulfonyl chloride [10], 4-fluoro7-nitrobenzo-2-oxa-1,3-diazole [11], and ion-exchange chromatography with postcolumn derivatization utilizing OPA [12] or ninhydrin [13,14].
Cystine was determined as cysteic acid and methionine as methionine sulfone after preoxidation with performic acid and precolumn derivation using phenylisothiocyanate (L-8800 Hitachi Automatic Amino Acid Analyzer, Tokyo, Japan).