phenylisothiocyanate


Also found in: Acronyms.

phen·yl·i·so·thi·o·cy·a·nate (PITC, PhNCS),

(fen'il-ī'sō-thī'ō-sī'ă-nāt),
A reagent that condenses with the free N-terminal amino group of a peptide chain to form a phenylthiohydantoin in the Edman method of identifying N-terminal amino acids.
Synonym(s): Edman reagent

Edman,

Pehr, Australian scientist, 1916-1977.
Edman method - a method of identifying N-terminal amino acids.
Edman reagent - used in the Edman method of identifying N-terminal amino acids Synonym(s): phenylisothiocyanate
References in periodicals archive ?
Prediction of reversed-phase liquid chromatography retention parameters for phenylisothiocyanate derivatives of amino acids.
Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate.
Amino acid analysis by reverse-phase high performance liquid chromatography: Pre-column derivatization with phenylisothiocyanate.
Amino acid analysis of physiological fluid by high-performance liquid chromatography with phenylisothiocyanate derivatization and comparison with ion-exchange chromatography.
Cystine was determined as cysteic acid and methionine as methionine sulfone after preoxidation with performic acid and pre-column derivation using phenylisothiocyanate (L-8800 Hitachi Automatic Amino Acid Analyzer, Tokyo, Japan).
The more usual methods involve precolumn derivatization with phenylisothiocyanate (PITC) (11,12) or o-phthalaldehyde (OPA) (7,13).
Several methods are suitable for the determination of amino acids (AAs) in biological fluids, including gas chromatography [1,2], reversed-phase chromatography with precolumn derivatization with various reagents such as orthophthalaldehyde (OPA) [3-6], 9-fluorenylmethyl-chloroformate [7], phenylisothiocyanate (PITC) [8, 9], dimethylaminonaphthalenesulfonyl chloride [10], dimethylaminoazobenzenesulfonyl chloride [10], 4-fluoro7-nitrobenzo-2-oxa-1,3-diazole [11], and ion-exchange chromatography with postcolumn derivatization utilizing OPA [12] or ninhydrin [13,14].
Cystine was determined as cysteic acid and methionine as methionine sulfone after preoxidation with performic acid and precolumn derivation using phenylisothiocyanate (L-8800 Hitachi Automatic Amino Acid Analyzer, Tokyo, Japan).
Cystine was determined as cysteic acid and methinonine as methionine sulfone after preoxidation with performic acid and precolumn derivation using phenylisothiocyanate (L-8800 Hitachi Automatic Amino Acid Analyzer, Tokyo, Japan).