Interestingly, Figure 5(f) shows that
phenylhydrazine treatment causes a progressive leak of hemoglobin through the membrane.
Acute intoxication with
phenylhydrazine on animals showed a significant inhibition of spontaneous rhythmic contractions of the thoracic duct: the frequency was reduced by 62.
5 mL), and MDA was derivatized with
phenylhydrazine (1 [micro]mol; 20 [micro]L of 50 mmol/L aqueous solution).
0 (Pearse, 1985; Bancroft & Cook), Acetylation + PAS (Pearse), acetylation + deacetylation + PAS (Pearse), acid hydrolysis + PAS (Quintarelli, 1961), periodic acid +
phenylhydrazine + Schiff reagent (Bancroft & Cook), periodic acid + thiosemicarbazide + Schiff reagent (Pearse; Bancroft & Cook) and observed in an Olympus BH-2 optical microscope.
Protective effect of the flavonoid silybin dihemisuccinate on the toxicity of
phenylhydrazine on rat liver.
Susceptibility of LDL to oxidation was tested by formation of thiobarbituric acid reacting substances (TBARS) after
phenylhydrazine exposure (16), and serum fatty acid profiles were determined by capillary gas chromatography (17).
Similarly
phenylhydrazine was reacted with enaminone 2 to afford 11b via intermediacy of 10b.
radical scavenging activity by DPPH reduction, superoxide radical scavenging activity in riboflavin/light/NBT system, nitric oxide (NO) radical scavenging activity in sodium nitroprusside/Greiss reagent system and inhibition of lipid peroxidation induced by iron-ADP-ascorbate in liver homogenate and
phenylhydrazine induced haemolysis in erythrocyte membrane stabilization study.
Seven acet ophenone-derived Schiff bases were synt hesized wit h different amines including 2-aminobenzoic acid (HL1) 4 -aminobenzoic acid (HL2) 2 -napht hylamin e (HL3)
phenylhydrazine (HL4) 12 -et hanediamine (HL5) 12 -diaminobenzene (HL6) and 14 - diaminobenzene (HL7) and were subject ed t o various assays including FRAP (ferric reducing ant ioxidant power) DPPH (11-diphenyl-2-picrylhydrazyl) phosphomolybdate reducing power and lipid peroxidat ion inhibit ion.
We added hydrazine monohydrate to the reaction mixture to trap cholest-4-ene-3-one, similar to the
phenylhydrazine reaction used to produce osazone from aldose (15).
The formazan derivatives in this study were prepared by the coupling reaction of hydrazones (obtained by the condensation reaction of m-nitro aldehyde and p-nitro aldehyde with
phenylhydrazine at pH 5.
Phenylhydrazine was used as prooxidant to treat fresh LDL samples in a two-step procedure: (a) LDL solutions (200 [micro]L) were incubated with 20 [micro]L of 0.
