model is an acute model for hemolytic anemia that lasts from 8 to 12 days and is considered a good, rapid tool for an investigational study.
We determined the oxidative effect of HBD degradation in HeLa shN and HeLa shR cells following phenylhydrazine
(PHZ) stimulation and overexpression of HBD.
Acute intoxication with phenylhydrazine
on animals showed a significant inhibition of spontaneous rhythmic contractions of the thoracic duct: the frequency was reduced by 62.74%, while the amplitude is decreased by 25.66% (p < 0.01).
Derivatization of MMDA and MDA with phenylhydrazine
yielded 4-methyl-1-phenylpyrazole and 1-phenylpyrazole, respectively.
McDonald, Insecticidal phenylhydrazine
8) uses phenylhydrazine
at 70 [degrees]C in an oxidizing medium on latex, which produces LNR with a molecular weight, according to time, of 3,000 to 20,000.
and other reagents/solvents of standard analytical grade were obtained from the same supplier.
Separate experiments were also performed in 5 mM [H.sub.2][O.sub.2] or 1 mM phenylhydrazine
Among these frequently used reagents are 2,4-dinitro- phenylhydrazine
[29-30], 4-hydrazine-2- stilbanzole , o-phenylenediamine [32, 33], 1,2- diamino-4,5-dimethoxybenzene , 1,2-diamino-4,5-methylenedioxyb enzene  and 4,5- diaminophthalhydrazide .
1, Dimethyl sulfoxide, cytochrome C, superoxide reductase, xanthinoxidase, xanthine, glutathione, o-phthaldialdehyde (OPT) and 2,4 dinitro phenylhydrazine
(DNPH) were purchased from Wako Pure Chemical Industries (Osaka, Japan).
For free MDA determination, 0.2 mL of plasma was diluted with 0.4 mol/L citric buffer (pH 4.0) added to 0.5 mmol/L butylated hydroxytoluene (5 nmol), 0.25 nmol of MDA-[d.sub.2] was then added, and the sample derivatized with 1 [micro]mol of phenylhydrazine
for 30 min at room temperature (final volume, 0.5 mL).
A well-known similar reaction takes place between some diamines, such as phenylhydrazine
, and compounds such as glucose or anhydroglucose.