Lack of 100% correlation between the vaginal biocoenosis test and cytological result according to the Bethesda system means that assessment of vaginal microflora in phase-contrast microscopy
should not be abandoned.
The approaches proposed in [14-16] were originally validated on another phase-contrast microscopy image dataset named C3H10T1/2, therefore we implemented the approaches and then applied them to the C2C12 dataset.
2] S Huh, DF Ker, R Bise, M Chen, and T Kanade, "Automated mitosis detection of stem cell populations in phase-contrast microscopy images," IEEE Transactions on Medical Imaging, vol.
13] S Huh, FEK Dai, H Su, and T Kanade, "Apoptosis Detection for Adherent Cell Populations in Time-Lapse Phase-Contrast Microscopy Images," in Proc.
In the case of quick quenching sample (H-Q), which the big difference was observed by the phase-contrast microscopy, at a first glance, there was no difference (especially regarding the size and number of spherical substances) between H-Q and H-QA observation by polarizing microscopy.
Figure 5a and b show the micrographs which were obtained by the polarizing microscopy and the phase-contrast microscopy of quite same cross sections of same H-Q sheet, respectively.
Figure 13 shows the micrographs obtained by phase-contrast microscopy of cross section of PP sheet cooled under different conditions (a) dry ice/ethyl alcohol, (b) ice water, and (c) 40[degrees]C water after melted on the hot stage.
9 mL of 1% ammonium oxalate solution; samples then were examined under phase-contrast microscopy
with an improved Neubauer hemocytometer (3) (Hawksley and Sons).
Thus, in our study we observed that of 131 samples with microhematuria by microscopy, 41 (31%) had predominantly dysmorphic erythrocytes according to both methods, whereas 59 (45%) had predominantly normal erythrocytes, but 16 (12%) revealed their dysmorphism only when analyzed by phase-contrast microscopy with a significant presence of altered cell shape (acanthocytes), and 15 (11%) showed their dysmorphism only when analyzed by flow cytometry, probably because of the presence of high amounts of yeasts or erythrocytes with different sizes.
In a laboratory of nephrology, however, where samples have a strong preselection, such an algorithm is not applicable and all samples must be analyzed by phase-contrast microscopy.
After 20 [micro]L of Alcian blue-pyronin B supravital stain was added to the urine (13), 1-[micro]L volumes were counted in disposable chambers (Fast-Read 10; Bio-Sigma) by both brightfield and phase-contrast microscopy
(Nikon Eclipse E400; Nikon Europe B.