phase-contrast microscopy


Also found in: Encyclopedia, Wikipedia.

phase-contrast microscopy

a microscopy technique for viewing colourless transparent objects by exploiting the interference between diffracted and undiffracted beams of light to produce an increase in contrast. When such an object is viewed through a light microscope the resulting image has a uniform brightness though the light emerging from different parts of the object has different phase relations which are due to variations in the refractive index. Phase-contrast microscopy uses these phase differences to give an image that would be produced had these differences been in the amplitude of the light.
Mentioned in ?
References in periodicals archive ?
A technical comparison of evaluating asbestos concentration by phase-contrast microscopy (PCM), scanning electron microscopy (SEM), and analytical transmission electron microscopy (ATEM) as illustrated from data generated from a case report.
Liu, "Dynamic phase-contrast microscopy of living cells by digital holography," Journal of Beijing University of Aeronautics & Astronautics, vol.
Kurzactkowski, "Ultrastructure of Candida albicans pleomorphic forms: Phase-contrast microscopy, scanning and transmission electron microscopy," Polish Journal of Microbiology, vol.
Lack of 100% correlation of vaginal biocoenosis test results with the cytological results according to the Bethesda system means that assessing the vaginal microflora in phase-contrast microscopy should not be abandoned.
There are several features which represent the characteristics of phase-contrast microscopy images, such as SIFT [4], GIST [5], etc.
The sensitivity of this technique can be further increased by using phase-contrast microscopy or examination at 400x magnification.
Lactate dehydrogenase (LDH) assay and phase-contrast microscopy were used to estimate quantitative and qualitative extent of the neuronal damage, respectively.
Figure 2 shows micrographs obtained by phase-contrast microscopy of cross section (a) H-SC, (b) H-Q, (c) HSCA, and (d) H-QA sheet samples.
This reprint of the original 2003 edition covers: elementary principles of phase-contrast microscopy, electron optics, wave optics, coherence and Fourier optics, STEM and Z-contrast, electron sources and detectors, measurement of electron-optical parameters, instabilities and the microscope environment, experimental methods, and associated techniques.
Trypan blue exclusion assay, phase-contrast microscopy, loss of cell adherence, and MTT assay were used to evaluate cadmium toxicity.
The upper reference limits for phase-contrast microscopy used in our laboratory are as follows: erythrocytes, <2/high-power field (HPF); leukocytes, <4/HPF; small-sized hyaline casts, <4 in each slide; hyaline with medium or large diameter, cellular, granular, granulo-hematic, waxy casts, none; cystine crystals, none; other crystals, rare (<10 in each slide).
These fractions were free of spores and aggregates of any kind, as observed by phase-contrast microscopy, SEM, and incubation on LB agar plates.

Full browser ?