phase-contrast microscopy


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phase-contrast microscopy

a type of light microscopy in which a special condenser and objective with a phase-shifting ring are used to visualize small differences in refractive index as differences in intensity or contrast. It is useful in viewing unstained specimens that appear transparent.

phase-contrast microscopy

a microscopy technique for viewing colourless transparent objects by exploiting the interference between diffracted and undiffracted beams of light to produce an increase in contrast. When such an object is viewed through a light microscope the resulting image has a uniform brightness though the light emerging from different parts of the object has different phase relations which are due to variations in the refractive index. Phase-contrast microscopy uses these phase differences to give an image that would be produced had these differences been in the amplitude of the light.
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Lack of 100% correlation between the vaginal biocoenosis test and cytological result according to the Bethesda system means that assessment of vaginal microflora in phase-contrast microscopy should not be abandoned.
The approaches proposed in [14-16] were originally validated on another phase-contrast microscopy image dataset named C3H10T1/2, therefore we implemented the approaches and then applied them to the C2C12 dataset.
2] S Huh, DF Ker, R Bise, M Chen, and T Kanade, "Automated mitosis detection of stem cell populations in phase-contrast microscopy images," IEEE Transactions on Medical Imaging, vol.
13] S Huh, FEK Dai, H Su, and T Kanade, "Apoptosis Detection for Adherent Cell Populations in Time-Lapse Phase-Contrast Microscopy Images," in Proc.
In the case of quick quenching sample (H-Q), which the big difference was observed by the phase-contrast microscopy, at a first glance, there was no difference (especially regarding the size and number of spherical substances) between H-Q and H-QA observation by polarizing microscopy.
Figure 5a and b show the micrographs which were obtained by the polarizing microscopy and the phase-contrast microscopy of quite same cross sections of same H-Q sheet, respectively.
Figure 13 shows the micrographs obtained by phase-contrast microscopy of cross section of PP sheet cooled under different conditions (a) dry ice/ethyl alcohol, (b) ice water, and (c) 40[degrees]C water after melted on the hot stage.
9 mL of 1% ammonium oxalate solution; samples then were examined under phase-contrast microscopy with an improved Neubauer hemocytometer (3) (Hawksley and Sons).
Thus, in our study we observed that of 131 samples with microhematuria by microscopy, 41 (31%) had predominantly dysmorphic erythrocytes according to both methods, whereas 59 (45%) had predominantly normal erythrocytes, but 16 (12%) revealed their dysmorphism only when analyzed by phase-contrast microscopy with a significant presence of altered cell shape (acanthocytes), and 15 (11%) showed their dysmorphism only when analyzed by flow cytometry, probably because of the presence of high amounts of yeasts or erythrocytes with different sizes.
In a laboratory of nephrology, however, where samples have a strong preselection, such an algorithm is not applicable and all samples must be analyzed by phase-contrast microscopy.
After 20 [micro]L of Alcian blue-pyronin B supravital stain was added to the urine (13), 1-[micro]L volumes were counted in disposable chambers (Fast-Read 10; Bio-Sigma) by both brightfield and phase-contrast microscopy (Nikon Eclipse E400; Nikon Europe B.

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