In order to label larval musculature, the solution with the secondary antibody also contained 1:500 phalloidin
conjugated to Alexa Fluor 488 (catalog no.
TGF-[beta]1 was from R&D Systems, Minneapolis, MN, USA, and 4'-6-diamidino-2-phenylindole (DAPI) and Alexa Fluor[R] 555 phalloidin
were from Sigma-Aldrich, Seelze.
The red fluorescence indicated cytoskeleton stained by rhodamine phalloidin
and the blue fluorescence indicated the cell nucleus stained by DAPI.
Immunocytochemical localization of RyRs in dissociated hippocampal neuronal cell cultures co-labeled with the F-actin label phalloidin
to identify actin-rich dendritic spines confirmed RyR immunoreactive puncta throughout the dendritic shafts of cultured hippocampal neurons (Figure 2B).
The control cells stained with rhodamine phalloidin
to visualize actin protein showed uniform staining pattern (figure 7a).
For this, the explants were incubated in a 1:300 dilution of Texas Red-X phalloidin
(Molecular Probes; Eugene, Ore.
A cytoskeleton will be assessed by immunofluorescent staining with Rhodamine phalloidin
5] integrin subunit to test for changes in Fn receptor or with rhodamine phalloidin
to test for changes in actin.
Because these substrates were optically transparent, the cells could be visualized by inverted transmission and by fluorescence microscopy, after fixing and staining with fluorescein-labeled phalloidin
Using actin-specific rhodamine phalloidin
as a fluorescent marker, 5-HT-treated cells were found to have fewer actin filaments at the cell periphery, increased stress fiber formation, and smoother, denser edges than control cells.
, which constitutes the main agent of phallotoxins, is a cyclic thermostable and easily dialyzable octapeptide, with a molecular mass of ~900 Da (2).
Through the implication of antisense oligonucleotides injected into the zygote stage, we are currently investigating the precise role AFAP--110 plays in the breakdown and reformation of the actin cytoskeleton in conjunction with phalloidin
, another known structural modifier.