PPAR[gamma] activity was first assayed by immunofluorescence: (A) cells were fixed, stained with phalloidin
(red), and counterstained with DAPI (blue); then (B) PPRE-H2B-eGFP fluorescence intensity was quantified.
Staining was performed using anti-type 2-capsule (green) and anti-pig CD169 primary antibodies (red), 647-conjugated phalloidin
(magenta) and DAPI (blue).
The cells were fixed at 1 h after seeding in DCL at various conditions and then visualized with DAPI and FITC-conjugated phalloidin
. TE-treated DCL showed a higher number of attached HLEC than SDS-treated DCL.
Texas Red-X phalloidin
(Thermo Fisher, Massachusetts, USA) was applied at a concentration of 1: 1000 and was incubated 30 min at RT to stain F-actin.
F-Actin was stained by FITC phalloidin
(green), and the cell nuclei were stained by DAPI (blue).
Based on the mechanisms of hepatoprotection of OA during liver injury induced by other small molecules like phalloidin
, CCl4 , and acetaminophen , we hypothesized that OA attenuated ConA-induced liver injury in a PPAR[alpha]- and JNK-dependent manner.
After rinsing 3 times with PBS, the cells were stained with TRITC phalloidin
(40734ES75, Yeasen, China) for 30 mins at room temperature in the dark.
The DPBS solution containing Rhodamine phalloidin
(Thermo Fisher Scientific, Waltham, MA) was added for 15 minutes.
For actin filament visualization, the blocking solution was removed and cells were incubated with 200 [micro]l rhodamine phalloidin
solution (0.2 [micro]g/ml) for 20 min at room temperature.
(a) Representative images of cells attached to substrata coated with low (1.0 [micro]g/mL), intermediate (10 [micro]g/mL), and high (100 [micro]g/mL) concentrations of collagen that were stained using TRITC- phalloidin
. Scale bar = 20 [micro]m.
Okuda, "Introduction of phalloidin
labeled with fluorescein isothiocyanate into living polymorphonuclear leukocytes by electroporation," Journal of Biochemical and Biophysical Methods, vol.
Transfected Jurkat-TAg cells were placed on poly-L-lysine-coated coverslips with or without 2 [micro]g/mL anti-CD3 Ab (OKT3; Biolegend) for 30 min at 37[degrees]C, fixed in 4% paraformaldehyde for 10 min at room temperature, and stained with Alexa Fluor 546 phalloidin
(Thermo Fisher Scientific) and 4',6'-diamidino-2phenylindole (DAPI) (DOJINDO) for 40 min at room temperature.