phage display


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phage display

n.
A method of using genetically modified bacteriophages to make new proteins and replicate selected proteins, enabling the engineering of antibodies and the development of new drugs.

phage display

A technique that fuses peptides to capsid proteins on the surface of a bacteriophage, which allows screening of libraries of phage-displayed peptides for binding to specific ligands. Identification of the DNA sequence of a selected phage identifies its peptide sequence.

phage display

the display of recombinant PEPTIDES/PROTEINS on the surface of a PHAGE, for example M13, so that displayed proteins can be identified by their interaction with other proteins. The technique involves cloning the gene for the protein in a special phage cloning vector, such that the cloned gene is fused with the gene for a phage coat protein. The gene fusion directs synthesis of a hybrid coat protein, when the vector is propagated in an appropriate bacterial host. Upon assembly of the phages, this protein is inserted into the phage coat and hence displayed on the surface of phage particles. The procedure normally involves the use of PHAGE DISPLAY LIBRARY and rapid screening and selection of desired proteins. Uses include the engineering of a wide range of specific ANTIBODIES.
References in periodicals archive ?
XOMA 031 is a naive human Fab phage display library with more than 300 billion clones covering the human antibody repertoire.
Academic and corporate chemists, biologists, and medical specialists describe the combinatorial technology phage display and its role in drug discovery.
Antibody to M13 phage is instrumental in the titration of M13 phages during phage display panning and in the detection of antigen-specific phages by means of their displayed antibody fragments, e.
XOMA's extensive antibody expertise includes phage display library construction, antibody discovery, optimization, preclinical and clinical development, regulatory affairs, and cGMP manufacturing.
The researchers' success demonstrates that antibody phage display can be used to select antibodies for pathogen detection--even where traditional methods have proved inadequate.
Phage display selection of peptides that affect prostate carcinoma cells attachment and invasion.
said Monday it has signed an agreement with Cambridge Antibody Technology (CAT) to license the British firm's antibody phage display libraries to Chugai.
Dyax has granted Amersham a non-exclusive license to its phage display affinity ligand discovery process for finding ligands for use in chromatography separations.
NASDAQ: DYAX) announced today, based on successful antibody discovery work during a research evaluation period, Bayer Pharma AG (Bayer) has executed a license to Dyax's antibody phage display technology for development and potential commercialization of therapeutic antibody candidates identified using the Dyax library.
Phage display to remain as the predominant antibody library technology in 2017 and beyond.