The assay combines a chemical marker that fluoresces with the test sample and an oxidizing agent, such as the peroxyl
radical, the hydroxyl radical, or metal ions.
By trapping peroxyl
free radicals, vitamin E protects membranes from lipid peroxidation.
And they're finding that a large group of usually colorful compounds, some of which are flavonoids, may be responsible for much of the antioxidant protection against peroxyl
radicals produced in the test tube.
Oxidation of the PUFA generates fatty acid radical that rapidly adds oxygen forming peroxyl
radicals, which can oxidize further PUFAs, producing lipid hydroperoxides that can break down to yet more radical species and to a wide range of compounds, notably aldehydes .
The purpose of the current study was to test whether the components of C4, individually or in combination, would produce reactive oxygen species including hydroxyl and peroxyl
in cultured cells.
This method is based on the scavenging activity of essential oils against peroxyl
radicals generated from the AAPH radical inductor.
Almeida., Action of Phenolic Derivatives (Acetaminophen, Salycilate and 5-Aminosalucilate) as Inhibitors of Membrane Lipid Peroxidation and as Peroxyl
Radical Scavengers, Arch.
A volume of 200 [micro]L from the total homogenate was used to perform the measurement of antioxidant capacity against peroxyl
radicals (ACAP), whereas 300 [micro]L was used to measure nitrite levels and 100 [micro]L to lipid peroxidation assay (LPO).
Evaluation of antioxidant properties of silymarin and its potential to inhibit peroxyl
radicals in vitro.
Supplementation of dietary components like vita-min E (tocopherols and tocorienols) lower lipid peroxi-dation (LPO), protein oxidation and the incidence of human morbidity and mortality.22 Vitamin E is the most efficient scavenger of lipid peroxyl
radicals.23 It interacts with the cell membrane, traps free radicals and inhibits ROS-induced generation of lipid peroxyl
radicals, thereby protecting the cells from peroxidation of polyunsaturated fatty acids in membrane phospho-lipids.24 Keeping in view the anti-oxidant role of vita-min E, the current investigation was designed to evalu-ate its impact on phthalate induced toxicity in testi-cular tissue of adult albino rats.
In general, the methods were divided into two major groups: Assays based on a single electron transfer (SET) reaction, with a change in color as the oxidant is reduced, and assays based on a hydrogen atom transfer (HAT) (Huang et al., 2005), which measures the activity of the antioxidant to scavenge the peroxyl
radicals, such as, the total radical trapping antioxidant parameter (TRAP) assay, the oxygen radical absorbance capacity (ORAC) assay, and the luminol-chemiluminescence-based peroxyl
radical scavenging capacity (LPSC) assay (Alho and Leinonen, 1999; Huang et al., 2005; Ou et al., 2001).
Exercise training is accompanied with induction of oxidative stress via over production overproduction of reactive oxygen species (ROS) including superoxide anion ([O.sup.2-]), hydroxyl (OHHO.) and peroxyl
radical (RO2.) (3).