HeLa cells and B-cells treated with anti-HeLa cells and anti-B-cells antibodies were devoid of peroxidase reaction product which indicated that the receptors were masked with antibodies.
The B cells and HeLa cells treated with anti-B cells antibodies and anti-HeLa cells antibodies respectively were devoid of peroxidase reaction product.
A positive peroxidase reaction
results in a reddish-brown precipitate, and a positive phenoloxidase reaction yields a blue-black deposit of melanin.
The peroxidase reaction
was developed for 10min with 3, 3'-diaminobenzidine (DAB).
The hydrogen peroxide formed after L-glutamate oxidase reaction can be easily detected by several methods used glutamate oxidase such as biosensor and the chromogenic peroxidase reaction
2] and 1,2 phenylendiamine were added and the amount of ML I was calculated after photometric quantification of the peroxidase reaction
at [lambda] = 492 nm.
0] temperatures and 20000 around to measure the ascorbate enzyme, 3 ml ascorbate peroxidase reaction
was contained ,50 mmol of potassium buffer with 7 PH, .
SUMMARY: The localization of peroxidase activity in different cell regions is used as a criterion for the classification of the stage of maturation of mammalian mononuclear phagocytes with a positive peroxidase reaction indicating the presence of monoblasts, promonocytes, monocytes and macrophages.
a large nucleus with proeminent nucleoli, a nucleocytoplasmatic ratio greater than 1, and the peroxidase reaction was localized in the cytoplasmatic granules (Fig.
The IgG was then visualized by the addition of 3-amino-9-ethyl-carbazole as chromogen in the peroxidase reaction
(examples of the resulting OCB patterns can be seen in Fig.
Because the blood reaction is based on the liberation of oxygen from peroxide by the peroxidase-like activity of heme, an inhibition of the peroxidase reaction
should cause a false-negative reaction.
Following a series of washes, the peroxidase reaction
was completed using diaminobenzidine with a nickel solution being added to achieve a black color.