peroxidase reaction

per·ox·i·dase re·ac·tion

formation of indophenol blue by the action of an oxidizing enzyme present in certain cells and tissues when they are treated with a solution of α-naphthol and dimethylparaphenylenediamine; by this method, cells of the myelocyte series, which give a positive reaction, may be distinguished from those of the lymphocyte series, which give a negative reaction.
Synonym(s): Nadi reaction
References in periodicals archive ?
2] and 1,2 phenylendiamine were added and the amount of ML I was calculated after photometric quantification of the peroxidase reaction at [lambda] = 492 nm.
0] temperatures and 20000 around to measure the ascorbate enzyme, 3 ml ascorbate peroxidase reaction was contained ,50 mmol of potassium buffer with 7 PH, .
The IgG was then visualized by the addition of 3-amino-9-ethyl-carbazole as chromogen in the peroxidase reaction (examples of the resulting OCB patterns can be seen in Fig.
The peroxidase reaction was carried out by using aminoethylcarbonate as the final chromogen.
Because the blood reaction is based on the liberation of oxygen from peroxide by the peroxidase-like activity of heme, an inhibition of the peroxidase reaction should cause a false-negative reaction.
Following a series of washes, the peroxidase reaction was completed using diaminobenzidine with a nickel solution being added to achieve a black color.
The peroxidase reaction was developed over approximately 3 min using 0.