peroxidase reaction

per·ox·i·dase re·ac·tion

formation of indophenol blue by the action of an oxidizing enzyme present in certain cells and tissues when they are treated with a solution of α-naphthol and dimethylparaphenylenediamine; by this method, cells of the myelocyte series, which give a positive reaction, may be distinguished from those of the lymphocyte series, which give a negative reaction.
Synonym(s): Nadi reaction
Farlex Partner Medical Dictionary © Farlex 2012
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The peroxidase reaction was stopped by the addition of 2 N [H.sub.2]S[O.sub.4], and absorbance at 450 nm was measured.
Within the confines of the RBC, autoxidation is minimized by the metHb reductase system; however free metHb can undergo a further peroxidase reaction with [O.sub.2.sup.*-] to generate ferrylHb ([Fe.sup.4+]) and the ROS hydrogen peroxide ([H.sub.2][O.sub.2]) that in turn attacks Hb to produce protein radicals, irreversibly damaging the Hb molecule leading to heme degradation and release of free iron [2].
HeLa cells and B-cells treated with anti-HeLa cells and anti-B-cells antibodies were devoid of peroxidase reaction product which indicated that the receptors were masked with antibodies.
A positive peroxidase reaction results in a reddish-brown precipitate, and a positive phenoloxidase reaction yields a blue-black deposit of melanin.
Based on this information it is obviously very important to develop specific analytical methods for measuring this amino acid preferably in simple and reliable way [6-13].The hydrogen peroxide formed after L-glutamate oxidase reaction can be easily detected by several methods used glutamate oxidase such as biosensor and the chromogenic peroxidase reaction [4 14-19].
After washing, [H.sub.2] [O.sub.2] and 1,2 phenylendiamine were added and the amount of ML I was calculated after photometric quantification of the peroxidase reaction at [lambda] = 492 nm.
5.0 grams of wheat leaf powder mixed with 10 ml of potassium buffer and centrifuged for 15 minutes with 4 [C.sup.0] temperatures and 20000 around to measure the ascorbate enzyme, 3 ml ascorbate peroxidase reaction was contained ,50 mmol of potassium buffer with 7 PH, .05 mmol ascorbic acid, a tenth mmol EDTA, and a tenth of ml enzyme and a tenth mmol hydrogen peroxidase.
The peroxidase reaction was developed over approximately 3 min using 0.05% diaminobenzidine and 0.01% [H.sub.2][O.sub.2] in PB with 1% ammonium nickel sulphate and 1% cobalt chloride.
The glutathione peroxidase reaction: Molecular basis of the antioxidant function of selenium in mammals.
The IgG was then visualized by the addition of 3-amino-9-ethyl-carbazole as chromogen in the peroxidase reaction (examples of the resulting OCB patterns can be seen in Fig.
Achieve peroxidase reaction with 0.02% diaminobenthizine, 0.02% [H.sub.2][O.sub.2], 50 mmol/L Tris-HCl buffer (pH 7.6).