Its stromata are always pulvinate with only a few perithecia
In case of perithecial development (Table II), traces of perithecia
were observed in response to maltose in the case of S1, S2, S3, Sw92.1 and Sw17.2, while N5, N6 and N7 showed a better response to glucose.
To identify the isolated fungus at genus level; morphological characters such as (color of mycelium mat, shape of perithecia
, arrangements of ascospores in the ascus) were studied under stereo-microscope (Table 3).
olive green to brown, ostiolate, globose to subglobose 260-360 x 260-320 [micro]m in size anchor to the substrate by rhizoids.
embedded in calcareous rock, thallus scant, white; involucrellum scant; spores 18-28 [micro]m x 9-14 [micro]m Verrucaria calciseda 2.
In February and May 2002, on roots and crowns of plants dead by black foot rot at Miraflores and Tabay, respectively, clusters of reddish to reddish brown perithecia
were commonly found (Figure 2).
A widespread species of calcareous substrates with a whitish thallus and partially immersed perithecia
But if made to wet, fungal grow was inhibited and after seven days at room temperature (20-24[grados]C) yielded numerous perithecia
. The fungus was then mounted in water and studied with a light microscope, measurements and drawings of the various structures were made and photographs also were taken on a Leitz microscope with an automatic camera, attempts to obtain the fungus in pure culture were unsuccessful [2, 3, 8, 9, 10, 11, 14, 15, 16, 17].
Mycelium characteristics were recorded after 13 days by examination for coremia and perithecia
formation, hyphal pigmentation, mycelial thickness and surface texture, production of aerial hyphae, and the character of the mycelium margin.
Description.--Fruiting bodies: Stromata brick-red at maturity, eventually turning brownish-black to black with age; hemispherical, 3-7 mm across, confluent forming extensive lobed crusts; surface papillate with protruding tips of perithecia
but ostiolar necks do not extend beyond the crust of the stroma; ostioles breaking exposing pore into perithecium.
The ascospores, which are formed in perithecia
after "fertilization" of the primary stroma by conidia of another mating type (White and Bultman 1987), are ejected into the air and may serve as means for horizontal transmission to new host plants (Western and Cavett 1959; Kohlmeyer and Kohlmeyer 1974; White and Morgan-Jones 1987).
fimicola was extracted from mature perithecia
by using CTAB method (Pietro et al., 1995).