By completing the anastomosis, CPB was restarted with the perfusion cannula
directly inserted into the graft.
Perfusion of the donor heart was recommenced immediately (0 4 l./min.) by connecting the arterial cannula to a coronary perfusion line, and as soon as the aorta had filled to displace the air, it was clamped distal to the perfusion cannula
so that the coronary arteries would be perfused.
Immediately upon completing the donor nephrectomy, the renal artery was intubated by the perfusion cannula
and secured in place using a vicryl Endoloop device (Ethicon US, LLC).
In the case of femoral cannulations, a dorsalis pedis (DP) or posterior tibial (PT) distal perfusion cannula
can be placed to promote perfusion to the lower extremity.
ECMO cannulation consisted of a 24 Fr cannula placed in a femoral vein, a 19 Fr cannula placed in the contralateral femoral artery, and a 10 Fr distal perfusion cannula
placed in the same femoral artery.